Université de Toulouse, Laboratoire de Biotechnologies Agroalimentaire et Environnementale, EA 4565, Institut Universitaire de Technologies, 24 rue d'Embaquès, F-32000, Auch, France.
Biofouling. 2012;28(3):305-14. doi: 10.1080/08927014.2012.673122.
This study aimed to characterize biofilms from the paper industry and evaluate the effectiveness of enzymatic treatments in reducing them. The extracellular polymeric substances (EPS) extracted from six industrial biofilms were studied. EPS were mainly proteins, the protein to polysaccharide ratio ranging from 1.3 to 8.6 depending on where the sampling point was situated in the paper making process. Eight hydrolytic enzymes were screened on a 24-h multi-species biofilm. The enzymes were tested at various concentrations and contact durations. Glycosidases and lipases were inefficient or only slightly efficient for biofilm reduction, while proteases were more efficient: after treatment for 24 h with pepsin, Alcalase® or Savinase®, the removal exceeded 80%. Savinase® appeared to be the most adequate for industrial conditions and was tested on an industrial biofilm sample. This enzyme led to a significant release of proteins from the EPS matrix, indicating its potential efficiency on an industrial scale.
本研究旨在对造纸工业生物膜进行特征描述，并评估酶处理在减少生物膜方面的效果。从六个工业生物膜中提取了细胞外聚合物物质 (EPS)。EPS 主要为蛋白质，蛋白质与多糖的比例根据采样点在造纸过程中的位置从 1.3 到 8.6 不等。在 24 小时多物种生物膜上筛选了八种水解酶。在不同浓度和接触时间下测试了这些酶。糖苷酶和脂肪酶对生物膜的减少效果不佳或仅有轻微效果，而蛋白酶的效果更好：用胃蛋白酶、Alcalase®或 Savinase®处理 24 小时后，去除率超过 80%。Savinase®似乎最适合工业条件，并在工业生物膜样品上进行了测试。该酶导致 EPS 基质中蛋白质的大量释放，表明其在工业规模上具有潜在的效率。
