Department of Restorative Dentistry, Periodontology, Endodontology, Preventive and Pediatric Dentistry, Dental School, University Medicine, Greifswald Rotgerberstr. 8, 17475, Greifswald, Germany.
Odontology. 2021 Oct;109(4):780-791. doi: 10.1007/s10266-021-00599-z. Epub 2021 Mar 19.
Peri-implantitis is caused by microbial contamination and biofilm formation on the implant surface. To achieve re-osseointegration, the microbes must be completely removed from the surface. Adjunctive to mechanical cleaning, chemical treatment with enzymes or other substances could optimise the treatment outcome. Therefore, we investigated the efficacy of different enzymes, a surfactant, and a chelator in destabilising dental polymicrobial biofilm. The biofilm destabilising effect of the glycosidases α-amylase, dextranase, DispersinB, and lysozyme, as well as the proteinase subtilisin A, and the nuclease Benzonase, the chelator EDTA, and the surfactant cocamidopropyl betaine were investigated on biofilms, inoculated with plaque on rough titanium discs. The test and the control solutions were incubated for 15 min at 36 °C on biofilms, and loosened biofilm mass was removed by shear stress with a shaker. Fluorescence-stained biofilms were microscopically analysed. Acceptable cell tolerability concentrations of test substances were determined by the MTT (tetrazolium dye) assay on the MG-63 cell line. A statistically significant biofilm destabilising effect of 10% was shown with lysozyme (2500 µg/ml).
种植体周围炎是由种植体表面的微生物污染和生物膜形成引起的。为了实现再骨整合，必须将微生物从表面完全清除。除了机械清洁外，用酶或其他物质进行化学处理可以优化治疗效果。因此，我们研究了不同酶、表面活性剂和螯合剂在破坏牙多微生物生物膜方面的功效。在接种有粗糙钛片上菌斑的生物膜上，研究了糖苷酶α-淀粉酶、葡聚糖酶、DisperinB 和溶菌酶，以及蛋白酶枯草杆菌蛋白酶 A 和核酸酶 Benzonase、螯合剂 EDTA 和表面活性剂椰油酰胺丙基甜菜碱对生物膜的破坏作用。将测试和对照溶液在 36°C 下孵育 15 分钟，然后用振荡器的剪切力去除松动的生物膜质量。用荧光染色生物膜进行显微镜分析。通过 MG-63 细胞系的 MTT（四唑染料）测定法确定了测试物质的可接受细胞耐受性浓度。溶菌酶（2500µg/ml）表现出 10%的统计学上显著的生物膜破坏作用。
