Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
J Am Chem Soc. 2012 Apr 18;134(15):6685-94. doi: 10.1021/ja211570a. Epub 2012 Apr 6.
3-Phenylthiophene-based water-soluble copolythiophenes (CPT1) were designed for colorimetric and fluorometric detection of lipopolysaccharide (LPS). The sensor (CPT1-C) shows a high selectivity to LPS in the presence of other negatively charged bioanalytes as well an extreme sensitivity with the detection limit at picomolar level, which is the lowest ever achieved among all synthetic LPS sensors available thus far. Significantly, the sensing interaction can be apparently observed by the naked eyes, which presents a great advantage for its practical applications. The appealing performance of sensor was demonstrated to originate from the multiple electrostatic and hydrophobic cooperative interactions, synergetic with signal amplification via the conformational change of the 3-phenylthiophene-based copolymer main chain. As a straightforward application, CPT1-C is capable of rapidly discriminating the Gram-negative bacteria (with LPS in the membrane) from Gram-positive bacteria (without LPS).
基于 3-苯基噻吩的水溶性共聚物（CPT1）被设计用于比色法和荧光法检测脂多糖（LPS）。该传感器（CPT1-C）在存在其他带负电荷的生物分析物的情况下对 LPS 表现出高选择性，以及对 LPS 的极端灵敏度，检测限达到皮摩尔级，这是迄今为止所有已报道的合成 LPS 传感器中最低的。重要的是，传感相互作用可以通过肉眼明显观察到，这为其实际应用提供了很大的优势。传感器的诱人性能源于多种静电和疏水协同相互作用，以及通过基于 3-苯基噻吩的共聚物主链构象变化进行信号放大。作为一种直接的应用，CPT1-C 能够快速区分革兰氏阴性菌（细胞膜中有 LPS）和革兰氏阳性菌（细胞膜中没有 LPS）。
