Rangin Marianne, Basu Amit
Department of Chemistry, Brown University, Providence, Rhode Island 02912, USA.
J Am Chem Soc. 2004 Apr 28;126(16):5038-9. doi: 10.1021/ja039822x.
The outer membrane of Gram negative bacteria contains lipopolysaccharides, which are glycolipids with carbohydrate sequences that are unique for each bacterial species and serotype. In this communication, we report a method for identifying LPS from different bacteria using an electronic tongue approach. Two functionalized polydiacetylene liposomes were used as colorimetric sensors for detecting various types of LPS. These liposomes were assayed under four different experimental conditions to generate a data set of eight colorimetric responses. This data set constitutes a unique fingerprint for each analyte and permits identification of each LPS type.
革兰氏阴性菌的外膜含有脂多糖，脂多糖是一种糖脂，其碳水化合物序列对每种细菌物种和血清型而言都是独一无二的。在本通讯中，我们报告了一种使用电子舌方法从不同细菌中鉴定脂多糖的方法。两种功能化的聚二乙炔脂质体被用作比色传感器来检测各种类型的脂多糖。这些脂质体在四种不同的实验条件下进行检测，以生成一组包含八种比色响应的数据集。该数据集构成了每种分析物的独特指纹，并允许识别每种脂多糖类型。
