溶组织内阿米巴转羧基酶的纯化和细胞定位。
Purification and cellular localization of the Entamoeba histolytica transcarboxylase.
机构信息
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340, Mexico, D.F., Mexico.
出版信息
Parasitol Res. 2012 Sep;111(3):1401-5. doi: 10.1007/s00436-012-2898-6. Epub 2012 Mar 28.
Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.
对溶组织内阿米巴的基因组分析预测了乙酰辅酶 A 羧化酶的存在。通过 Western blot、组织化学和共聚焦显微镜，我们证明了溶组织内阿米巴细胞质中存在一种含有生物素的蛋白质，分子量为 136 kDa，具有生物素羧化酶活性。该蛋白可能对应一种转羧酶，它催化限速反应，导致脂肪酸延长。
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