Disterer Petra, Khoo Bernard
Department of Endocrinology, UCL Medical School, London, UK.
Methods Mol Biol. 2012;867:289-305. doi: 10.1007/978-1-61779-767-5_19.
Exon-skipping antisense oligonucleotides (ASOs) can be used to knockdown the expression of an undesired gene or specific gene isoform. This chapter discusses the potential therapeutic applications of the technique and provides a sample protocol for inducing exon-skipping in Apolipoprotein B in vitro, as well as a protocol for quantifying exon-skipping using real-time PCR.
外显子跳跃反义寡核苷酸（ASO）可用于降低不期望基因或特定基因亚型的表达。本章讨论了该技术的潜在治疗应用，并提供了一个在体外诱导载脂蛋白B外显子跳跃的示例方案，以及一个使用实时PCR定量外显子跳跃的方案。
