Weissmann G, Hoffstein S, Korchak H, Smolen J E
Trans Assoc Am Physicians. 1978;91:90-103.
The two findings reported here clearly indicate that the human granulocyte undergoes stimulus-secretion coupling when appropriate stimuli engage the cell surface. These secretory and metabolic responses seem designed to provide efficient mechanisms for bacterial killing or the elimination of immune complexes. First, ligands bind to surface receptors, in the case of concanavalin A, via surface sugars, in the case of immune complexes, via Fc receptors. Our new findings, described above, show that this is followed within five to ten seconds by membrane hyperpolarization: changes in the membrane potential of the cell: delta psi. Local loss of membrane calcium is noted concurrently: not as part of a generalized "triggering" of all the surface membraneof the cell, but only at the point of ligand-receptor interaction: the initiating point of phagocytosis. The subsequent changes, such as superoxide anion production, the generation of thromboxanes and prostaglandins, and the intracellular events which lead to secretion appear, therefore, to be secondary to these earliest membrane responses of phagocytosis.
此处报道的两项研究结果清楚地表明，当适当的刺激作用于细胞表面时，人类粒细胞会发生刺激-分泌偶联。这些分泌和代谢反应似乎旨在为细菌杀伤或免疫复合物清除提供有效的机制。首先，配体与表面受体结合，在伴刀豆球蛋白A的情况下，通过表面糖结合；在免疫复合物的情况下，通过Fc受体结合。我们上述的新发现表明，在此后的五到十秒内会发生膜超极化：细胞的膜电位变化：Δψ。同时会注意到膜钙的局部丢失：这并非细胞所有表面膜普遍“触发”的一部分，而仅发生在配体-受体相互作用的位点：吞噬作用的起始点。因此，随后的变化，如超氧阴离子的产生、血栓烷和前列腺素的生成，以及导致分泌的细胞内事件，似乎继发于吞噬作用这些最早的膜反应。
