Centre de Recherche en Photonique, Département d'informatique et d'ingénierie, Université du Québec en Outaouais, Gatineau, QC, J8Y 3G5, Canada.
Centre de Recherche en Photonique, Département d'informatique et d'ingénierie, Université du Québec en Outaouais, Gatineau, QC, J8Y 3G5, Canada.
Biosens Bioelectron. 2012 May 15;35(1):308-312. doi: 10.1016/j.bios.2012.03.006. Epub 2012 Mar 10.
In this paper we report a stable, label-free, bacteriophage-based detection of Escherichia coli (E. coli) using ultra sensitive long-period fiber gratings (LPFGs). Bacteriophage T4 was covalently immobilized on optical fiber surface and the E. coli binding was investigated using the highly accurate spectral interrogation mechanism. In contrast to the widely used surface plasmon resonance (SPR) based sensors, no moving part or metal deposition is required in our sensor, making the present sensor extremely accurate, very compact and cost effective. We demonstrated that our detection mechanism is capable of reliable detection of E. coli concentrations as low as 10(3)cfu/ml with an experimental accuracy greater than 99%.
本文报道了一种基于噬菌体的稳定、无标记的大肠杆菌（E. coli）检测方法，该方法使用超灵敏长周期光纤光栅（LPFGs）。噬菌体 T4 被共价固定在光纤表面，并使用高度精确的光谱检测机制研究了其与大肠杆菌的结合。与广泛使用的基于表面等离子体共振（SPR）的传感器不同，我们的传感器不需要移动部件或金属沉积，因此该传感器具有极高的准确性、非常紧凑且具有成本效益。我们证明了我们的检测机制能够可靠地检测低至 10(3)cfu/ml 的大肠杆菌浓度，实验精度大于 99%。
