School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, Hubei, China.
College of Life Science, Xinyang Normal University, Xinyang, Henan, China.
J Biophotonics. 2018 Sep;11(9):e201800012. doi: 10.1002/jbio.201800012. Epub 2018 May 10.
Escherichia coli bacteria have been found to be responsible for various health outbreaks caused by contaminated food and water. Accurate and rapid test of E. coli is thus crucial for protecting the public health. A fast-response, label-free bacteriophage-based detection of E. coli using multimode microfiber probe is proposed and demonstrated in this article. Due to the abrupt taper and subwavelength diameter, different modes are excited and guided in the microfiber as evanescent field that can interact with surrounding E. coli directly. The change of E. coli concentration and corresponding binding of E. coli bacteria on microfiber surface will lead to the shift of optical spectrum, which can be exploited for the application of biosensing. The proposed method is capable of reliable detection of E. coli concentration as low as 10 cfu/mL within the range of 10 to 10 cfu/mL. Owing to the advantages of high sensitivity and fast response, the microfiber probe has great potential application in the fields of environment monitoring and food safety.
已经发现大肠杆菌细菌是导致受污染食物和水引起的各种健康爆发的原因。因此，准确快速地检测大肠杆菌对于保护公众健康至关重要。本文提出并演示了一种使用多模微光纤探针的快速响应、无标签的基于噬菌体的大肠杆菌检测方法。由于急剧的锥形和亚波长直径，不同模式在微光纤中作为消逝场被激发和引导，可直接与周围的大肠杆菌相互作用。大肠杆菌浓度的变化以及大肠杆菌在微光纤表面上的相应结合将导致光谱的移动，这可用于生物传感的应用。所提出的方法能够可靠地检测低至 10 cfu/mL 的大肠杆菌浓度，检测范围为 10 至 10 cfu/mL。由于具有高灵敏度和快速响应的优点，微光纤探针在环境监测和食品安全等领域具有很大的潜在应用。
