Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Cont Lens Anterior Eye. 2012 Aug;35(4):155-62. doi: 10.1016/j.clae.2012.02.006. Epub 2012 Mar 26.
Prevention of Pseudomonas aeruginosa binding to soft-contact lenses (SCLs) may curtail sight-threatening microbial keratitis. Substrate surface wettability is known to modulate adhesion of P. aeruginosa. This study investigates the use of aqueous alkoxylate block co-polymer surfactants for enhanced wettability and antibacterial adhesion of SCLs under leaching conditions. Specifically, Pluronic(®) F127 (PF) and three ethylene oxide-butylene oxide (EOBO) surfactants were studied with four commercially available silicone-hydrogel contact lenses: Pure Vision™, Acuvue Advance™, Acuvue Oasys™ and O(2)Optix™. Dilute aqueous PF and EOBO surfactants impregnated all four soft-contact lenses, as demonstrated by surface-tension decline for leached surfactant. For PF surfactant, significant surface-wettability improvement upon rinsing occurred only after overnight leaching. EOBO surfactant showed a similar pattern with O(2)Optix™ lenses. EOBO-pretreated Pure Vision™ lenses, however, showed fast leaching and a significant change in surface energy towards improved wettability. Adhesion assays of P. aeruginosa displayed a small decrease in the binding rate of PAK bacteria for EOBO-pretreated Pure Vision™ lenses, but not for EOBO-pretreated O(2)Optix™ lenses. P. aeruginosa strain-PAO1 bacteria adhesion to all lenses was independent of surface wettability. Despite the ability of polymeric surfactants to lower advancing contact angles under leaching conditions, increased lens wettability is not a universal panacea for antifouling of soft-contact lenses.
预防铜绿假单胞菌与软性隐形眼镜（SCL）结合，可能会减少威胁视力的微生物角膜炎。已知基底表面润湿性可调节铜绿假单胞菌的粘附。本研究调查了在浸出条件下，使用水性烷氧基化嵌段共聚物表面活性剂提高 SCL 的润湿性和抗菌粘附性。具体而言，研究了 Pluronic（®）F127（PF）和三种氧化乙烯-氧化丙烯（EOBO）表面活性剂与四种市售硅水凝胶隐形眼镜：Pure Vision™、Acuvue Advance™、Acuvue Oasys™和 O（2）Optix™。稀释的 PF 和 EOBO 表面活性剂浸渍所有四种软性隐形眼镜，这可以通过浸出表面活性剂的表面张力下降来证明。对于 PF 表面活性剂，仅在过夜浸出后冲洗时才会显著改善表面润湿性。EOBO 表面活性剂在 O（2）Optix™镜片上显示出类似的模式。然而，EOBO 预处理的 Pure Vision™镜片表现出快速浸出和表面能的显著变化，从而提高了润湿性。铜绿假单胞菌的粘附实验显示，经过 EOBO 预处理的 Pure Vision™镜片的 PAK 细菌结合率略有下降，但经过 EOBO 预处理的 O（2）Optix™镜片没有下降。所有镜片上铜绿假单胞菌 PAO1 细菌的粘附与表面润湿性无关。尽管聚合物表面活性剂在浸出条件下能够降低前进接触角，但增加镜片润湿性并不是软性隐形眼镜防污的万能方法。
