基于连接反应与四链体形成的无标记荧光 DNA 探针用于烟酰胺腺嘌呤二核苷酸的高灵敏和选择性检测
A label-free fluorescence DNA probe based on ligation reaction with quadruplex formation for highly sensitive and selective detection of nicotinamide adenine dinucleotide.
机构信息
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
出版信息
Chem Commun (Camb). 2012 May 11;48(37):4468-70. doi: 10.1039/c2cc30540b. Epub 2012 Mar 29.
PMID:22456321
Abstract
A simple label-free fluorescent sensing scheme for sensitive and selective detection of nicotinamide adenine dinucleotide (NAD(+)) has been developed based on DNA ligation reaction with ligand-responsive quadruplex formation. This approach can detect 0.5 nM NAD(+) with high selectivity against other NAD(+) analogs.
摘要
基于配体响应的四链体形成的 DNA 连接反应，开发了一种简单的无标记荧光传感方案，用于灵敏和选择性检测烟酰胺腺嘌呤二核苷酸（NAD(+)）。该方法可以检测到 0.5 nM 的 NAD(+)，对其他 NAD(+)类似物具有高选择性。
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