Área de Neurociencias, Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México, México.
Neuropsychobiology. 2012;65(3):161-7. doi: 10.1159/000330010. Epub 2012 Mar 27.
Rapid eye movement (REM) sleep is involved in memory consolidation, which implies synaptic plasticity. This process requires protein synthesis and the reorganization of the neural cytoskeleton. REM sleep deprivation (REMSD) has an impact on some neuronal proteins involved in synaptic plasticity, such as glutamate receptors and postsynaptic density protein 95, but its effects on cytoskeletal proteins is unknown. In this study, the effects of REMSD on the content of the cytoskeletal proteins MAP2 and TAU were analyzed. Adult female rats were submitted to selective REMSD by using the multiple platform technique. After 24, 48 or 72 h of REMSD, rats were decapitated and the following brain areas were dissected: pons, preoptic area, hippocampus and frontal cortex. Protein extraction and Western blot were performed. Results showed an increase in TAU content in the pons, preoptic area and hippocampus after 24 h of REMSD, while in the frontal cortex a significant increase in TAU content was observed after 72 h of REMSD. A TAU content decrease was observed in the hippocampus after 48 h of REMSD. Interestingly, a marked increase in TAU content was observed after 72 h of REMSD. MAP2 content only increased in the preoptic area at 24 h, and in the frontal cortex after 24 and 72 h of REMSD, without significant changes in the pons and hippocampus. These results support the idea that REM sleep plays an important role in the organization of neural cytoskeleton, and that this effect is tissue-specific.
快速眼动（REM）睡眠参与记忆巩固，这意味着突触可塑性。这个过程需要蛋白质合成和神经细胞骨架的重组。REM 睡眠剥夺（REMSD）会影响一些参与突触可塑性的神经元蛋白，如谷氨酸受体和突触后密度蛋白 95，但它对细胞骨架蛋白的影响尚不清楚。在这项研究中，分析了 REMSD 对细胞骨架蛋白 MAP2 和 TAU 含量的影响。成年雌性大鼠通过使用多平台技术进行选择性 REMSD。在 REMSD 24、48 或 72 小时后，大鼠断头并解剖以下脑区：桥脑、视前区、海马体和额叶皮质。进行蛋白质提取和 Western blot。结果显示，在 REMSD 24 小时后，桥脑、视前区和海马体中的 TAU 含量增加，而在额叶皮质中，在 REMSD 72 小时后，TAU 含量显著增加。在 REMSD 48 小时后，海马体中的 TAU 含量下降。有趣的是，在 REMSD 72 小时后，TAU 含量明显增加。MAP2 含量仅在 REMSD 24 小时后增加，在 REMSD 24 和 72 小时后增加，桥脑和海马体没有明显变化。这些结果支持 REM 睡眠在神经细胞骨架组织中起着重要作用的观点，并且这种作用是组织特异性的。
