Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa.
Biotechnol Lett. 2012 Jul;34(7):1351-6. doi: 10.1007/s10529-012-0909-4. Epub 2012 Mar 29.
Nicotiana tabacum cell suspensions, 2 g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 4'-hexopyranosyloxy-3'-methoxyisonitrosoacetophenone in 54 % yield over 18 h. Unconverted INAP was at 33 μM. UPLC-MS/MS analyses with MassFragment software were used for metabolite identification. INAP had been hydroxylated at its meta- and para-positions as well as undergoing subsequent methoxylation and glycosylation. INAP is thus recognized by the enzymatic machinery of the phenylpropanoid pathway and is converted to a molecule with a substitution pattern similar to ferulic acid.
烟草细胞悬浮液（2 g 湿重/毫升）能迅速摄取 1mM 的异亚硝基苯乙酮（INAP），这是一种植物来源的应激代谢物，具有抗氧化和抗真菌特性，在 18 小时内产生 4'-己糖氧基-3'-甲氧基异亚硝基苯乙酮，产率为 54%。未转化的 INAP 为 33μM。采用 UPLC-MS/MS 分析和 MassFragment 软件进行代谢物鉴定。INAP 在间位和对位被羟化，随后进行甲氧基化和糖基化。因此，INAP 被苯丙烷途径的酶机制识别，并转化为一种具有与阿魏酸类似取代模式的分子。
