da Camara Nikita, Dubery Ian A, Piater Lizelle A
Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg P.O. Box 524, South Africa.
Plants (Basel). 2023 Mar 2;12(5):1137. doi: 10.3390/plants12051137.
Proteins play an essential regulatory role in the innate immune response of host plants following elicitation by either biotic or abiotic stresses. Isonitrosoacetophenone (INAP), an unusual oxime-containing stress metabolite, has been investigated as a chemical inducer of plant defence responses. Both transcriptomic and metabolomic studies of various INAP-treated plant systems have provided substantial insight into this compound's defence-inducing and priming capabilities. To complement previous 'omics' work in this regard, a proteomic approach of time-dependent responses to INAP was followed. As such, () cell suspensions were induced with INAP and changes monitored over a 24-h period. Protein isolation and proteome analysis at 0, 8, 16 and 24 h post-treatment were performed using two-dimensional electrophoresis followed by the gel-free eight-plex isobaric tags for relative and absolute quantitation (iTRAQ) based on liquid chromatography and mass spectrometry. Of the identified differentially abundant proteins, 125 were determined to be significant and further investigated. INAP treatment elicited changes to the proteome that affected proteins from a wide range of functional categories: defence, biosynthesis, transport, DNA and transcription, metabolism and energy, translation and signalling and response regulation. The possible roles of the differentially synthesised proteins in these functional classes are discussed. Results indicate up-regulated defence-related activity within the investigated time period, further highlighting a role for proteomic changes in priming as induced by INAP treatment.
在受到生物或非生物胁迫诱导后，蛋白质在宿主植物的先天免疫反应中发挥着至关重要的调节作用。异亚硝基苯乙酮（INAP）是一种不同寻常的含肟胁迫代谢产物，已被作为植物防御反应的化学诱导剂进行研究。对各种经INAP处理的植物系统进行的转录组学和代谢组学研究，为该化合物的防御诱导和引发能力提供了大量深入见解。为补充此前在这方面的“组学”研究工作，采用了蛋白质组学方法来研究对INAP的时间依赖性反应。具体而言，用INAP诱导（）细胞悬浮液，并在24小时内监测变化。在处理后0、8、16和24小时进行蛋白质分离和蛋白质组分析，采用二维电泳，随后基于液相色谱和质谱的无胶八重等压标签相对和绝对定量（iTRAQ）方法。在鉴定出的差异丰富蛋白质中，确定有125种具有显著性，并对其进行了进一步研究。INAP处理引起了蛋白质组的变化，这些变化影响了来自广泛功能类别的蛋白质：防御、生物合成、运输、DNA和转录、代谢和能量、翻译以及信号传导和反应调节。讨论了这些功能类别中差异合成蛋白质的可能作用。结果表明，在所研究的时间段内与防御相关的活性上调，进一步突出了蛋白质组变化在INAP处理诱导的引发中的作用。
