School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
Arch Virol. 2012 Jul;157(7):1253-60. doi: 10.1007/s00705-012-1292-0. Epub 2012 Mar 29.
The complete genomes of tobamovirus isolates from Plantago major L. from New Zealand (NZ-439), Plantago sp. from Germany (Kons 1105), Actinidia chinensis (Actinidia-AC) and A. deliciosa (Actinidia-AD) from China were sequenced and compared to previously published tobamovirus genomes. Their genome organization and phylogenetic analysis of the putative replicase component, replicase readthrough component, movement protein, coat protein and complete genome placed all four isolates in subgroup 3 of the tobamoviruses. The complete genomes differed from each other by <8.5% and from published sequences of turnip vein clearing virus and youcai mosaic virus by about 12-13% and 19-20%, respectively. The aa sequences of the individual ORFs of the Plantago and Actinidia isolates differed from each other by <4% and were most similar to published (partial) sequences of ribgrass mosaic virus (RMV). We propose that these sequences constitute the first complete published sequences for RMV.
从新西兰（NZ-439）的车前草中、德国的车前草（Kons 1105）中、中国的猕猴桃（Actinidia chinensis，Actinidia-AC）和美味猕猴桃（A. deliciosa，Actinidia-AD）中分离出的豇豆花叶病毒分离株的完整基因组被测序，并与以前发表的豇豆花叶病毒基因组进行了比较。它们的基因组组织和假定复制酶成分、复制酶通读成分、运动蛋白、外壳蛋白和完整基因组的系统发育分析将这四个分离株都归入豇豆花叶病毒亚组 3。完整基因组彼此之间的差异<8.5%，与已发表的芜菁叶脉褪绿病毒和羽衣甘蓝花叶病毒序列的差异约为 12-13%和 19-20%。车前草和猕猴桃分离株的各个 ORF 的 aa 序列彼此之间的差异<4%，与已发表的（部分）黑麦草花叶病毒（RMV）序列最为相似。我们提出这些序列构成了 RMV 的第一个完整发表的序列。
