D.I. Ivanovsky Institute of Virology, Gamaleya Str. 16, Moscow 123098, Russia.
Virus Res. 2012 Jun;166(1-2):61-7. doi: 10.1016/j.virusres.2012.03.003. Epub 2012 Mar 21.
A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 amino acid positions recognized by monoclonal antibodies: 129, 156, 158, 159, and 190 (H3 numbering). The amino acid positions were distributed in two epitopes belonging to antigenic sites Sa and Sb. The mutant HAs exhibited variations in the affinity to synthetic high molecular mass sialic acid-containing receptor analogues. Results are discussed in connection with the antigenic drift potential of the "swine-like" pandemic 2009 influenza virus.
一个由 6 种针对 A/Moscow/IIV01/2009（H1N1）病毒的中和单克隆抗体（MAbs）组成的小组在 2009 年大流行期间被用来选择 26 种逃逸突变体。这些突变体在与单抗的免疫交叉反应中得到了表征。突变 HA 基因的测序显示了 5 个被单克隆抗体识别的氨基酸位置：129、156、158、159 和 190（H3 编号）。这些氨基酸位置分布在属于抗原表位 Sa 和 Sb 的两个表位中。突变的 HAs 在对合成高分子质量含唾液酸的受体类似物的亲和力上表现出差异。结果与“猪样”2009 年流感大流行病毒的抗原漂移潜力有关。
