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甲型 H1N1pdm09 流感病毒抗原突变株逃避人源单克隆抗体的多样性。

Diversity of antigenic mutants of influenza A(H1N1)pdm09 virus escaped from human monoclonal antibodies.

机构信息

Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.

Yokohama City Institute of Public Health, Yokohama, Japan.

出版信息

Sci Rep. 2017 Dec 18;7(1):17735. doi: 10.1038/s41598-017-17986-8.

DOI:10.1038/s41598-017-17986-8
PMID:29255273
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5735164/
Abstract

Since the 2017 Southern Hemisphere influenza season, the A(H1N1)pdm09-like virus recommended for use in the vaccine was changed because human, but not ferret, sera distinguish A(H1N1)pdm09 viruses isolated after 2013 from the previously circulating strains. An amino acid substitution, lysine to glutamine, at position 166 (H3 numbering) in the major antigenic site of HA was reported to be responsible for the antigenic drift. Here, we obtained two anti-A(H1N1)pdm09 HA monoclonal antibodies that failed to neutralize viruses isolated after 2013 from a vaccinated volunteer. Escape mutations were identified at position 129, 165, or 166 in the major antigenic site of HA. Competitive growth of the escape mutant viruses with the wild-type virus revealed that some escape mutants possessing an amino acid substitution other than K166Q showed superior growth to that of the wild-type virus. These results suggest that in addition to the K166Q mutation that occurred in epidemic strains, other HA mutations can confer resistance to antibodies that recognize the K166 area, leading to emergence of epidemic strains with such mutations.

摘要

自 2017 年南半球流感季节以来，疫苗中推荐使用的 A(H1N1)pdm09 样病毒发生了变化，因为人类而非雪貂血清能够区分 2013 年后分离的 A(H1N1)pdm09 病毒与先前流行的毒株。据报道，HA 主要抗原位点的一个氨基酸取代，即 166 位的赖氨酸到谷氨酰胺（H3 编号），导致了抗原漂移。在这里，我们从接种疫苗的志愿者中获得了两种无法中和 2013 年后分离的 A(H1N1)pdm09 病毒的抗 HA 单克隆抗体。在 HA 的主要抗原位点，129、165 或 166 位发生了逃逸突变。逃逸突变病毒与野生型病毒的竞争生长表明，一些具有除 K166Q 以外的氨基酸取代的逃逸突变体显示出比野生型病毒更高的生长优势。这些结果表明，除了在流行株中发生的 K166Q 突变外，其他 HA 突变可以赋予对识别 K166 区域的抗体的抗性，导致具有这些突变的流行株的出现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/0962086c1381/41598_2017_17986_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/3d620f8e0b9d/41598_2017_17986_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/c844321394cc/41598_2017_17986_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/885841e98cda/41598_2017_17986_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/b3378328e434/41598_2017_17986_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/0962086c1381/41598_2017_17986_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/3d620f8e0b9d/41598_2017_17986_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/c844321394cc/41598_2017_17986_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/885841e98cda/41598_2017_17986_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/b3378328e434/41598_2017_17986_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b906/5735164/0962086c1381/41598_2017_17986_Fig5_HTML.jpg

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本文引用的文献

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2
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J Infect Dis. 2016 Dec 15;214(12):1947-1951. doi: 10.1093/infdis/jiw479. Epub 2016 Oct 7.
3
猪流感疫苗接种会导致猪流感病毒实验感染时的不同漂移进化模式，并降低基因组重配的可能性。
Front Cell Infect Microbiol. 2023 Mar 13;13:1111143. doi: 10.3389/fcimb.2023.1111143. eCollection 2023.
4
A broadly protective human monoclonal antibody targeting the sialidase activity of influenza A and B virus neuraminidases.一种广泛保护性的人源单克隆抗体，针对甲型和乙型流感病毒神经氨酸酶的唾液酸酶活性。
Nat Commun. 2022 Nov 3;13(1):6602. doi: 10.1038/s41467-022-34521-0.
5
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Sci Rep. 2022 Sep 2;12(1):14971. doi: 10.1038/s41598-022-18977-0.
6
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Science. 2021 May 14;372(6543):738-741. doi: 10.1126/science.abf6648. Epub 2021 Apr 12.
7
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8
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Fatal Cases of Seasonal Influenza in Russia in 2015-2016.
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4
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Euro Surveill. 2016;21(11):30168. doi: 10.2807/1560-7917.ES.2016.21.11.30168.
5
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6
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Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):15798-803. doi: 10.1073/pnas.1409171111. Epub 2014 Oct 20.
7
Epitope mapping of the hemagglutinin molecule of A/(H1N1)pdm09 influenza virus by using monoclonal antibody escape mutants.利用单克隆抗体逃逸突变体对甲型（H1N1）pdm09流感病毒血凝素分子进行表位作图。
J Virol. 2014 Nov;88(21):12364-73. doi: 10.1128/JVI.01381-14. Epub 2014 Aug 13.
8
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9
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10
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Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9047-52. doi: 10.1073/pnas.1118979109. Epub 2012 May 21.