Special Education Collage, Changchun University, Changchun 130022, PR China.
Int J Biol Macromol. 2012 May 1;50(4):1075-80. doi: 10.1016/j.ijbiomac.2012.03.006. Epub 2012 Mar 19.
A water-soluble polysaccharide (FUP-1) was obtained from Fritillaria ussuriensis Maxim through warm water extraction, ethanol precipitation, anion-exchange and gel-permeation chromatography. Structural analysis of this compound revealed that the average molecular weight of FUP-1 was 4.1×10(4)Da, and the major monosaccharide component was xylose, followed by glucose and galactose, at low levels. In vitro antioxidant assays showed that FUP-1 exhibited strong hydroxyl, superoxide anion and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activities. During in vivo antioxidant testing, the administration of FUP-1 could largely attenuate the increase in d-gal-induced malondialdehyde (MDA) and renew the activities of superoxide dismutase (SOD) in serums as well as glutathione peroxidase (GSH-Px) and the total antioxidant capacity (T-AOC) in the livers of d-gal-treated mice. Overall, the results of the present study indicate that FUP-1 exerts antioxidant activity not only through its own radical-scavenging activity but also by boosting the enzymatic and nonenzymatic antioxidant defense system of the host.
从乌头中提取出一种水溶性多糖（FUP-1）通过温水萃取、乙醇沉淀、阴离子交换和凝胶渗透色谱。该化合物的结构分析表明，FUP-1 的平均分子量为 4.1×10（4）Da，主要单糖组分为木糖，其次是葡萄糖和半乳糖，含量较低。体外抗氧化试验表明，FUP-1 具有较强的羟基、超氧阴离子和 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性。在体内抗氧化试验中，FUP-1 的给药可显著降低 d-半乳糖诱导的丙二醛（MDA）的增加，并恢复血清中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（T-AOC）的活性在 d-半乳糖处理的小鼠肝脏中。总的来说，本研究的结果表明，FUP-1 不仅通过自身的自由基清除活性，而且通过增强宿主的酶和非酶抗氧化防御系统发挥抗氧化活性。
