Lee S E, Ju E M, Kim J H
Department of Oral Biochemistry, College of Dentistry, Kyung Hee University, Seoul, Korea.
Exp Mol Med. 2001 Dec 31;33(4):263-8. doi: 10.1038/emm.2001.43.
The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol extract (Me) revealed the presence of high 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50 7.4 microg/ml) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between 4-100 microg/ml. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.
菝葜根提取物已被用作药物，并据报道具有抗菌和抗诱变活性。在本研究中，对菝葜根提取物可能存在的抗氧化活性进行了研究。甲醇提取物（Me）显示出高1,1-二苯基-2-苦基肼（DPPH）自由基清除活性（IC50为7.4微克/毫升）以及对细胞活力的保护作用。通过各种溶剂萃取和测定进一步分级分离表明，乙酸乙酯、丁醇和水萃取部分具有高水平的DPPH自由基清除活性。此外，用菝葜根的Me处理的V79-4细胞在4-100微克/毫升之间以剂量依赖的方式诱导超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性增加。这些结果表明，菝葜根提取物的药用成分也含有抗氧化活性。
