Civil Engineering, College of Engineering and Informatics and Ryan Institute, National University of Ireland, Galway, Ireland.
Bioresour Technol. 2012 Jun;114:406-13. doi: 10.1016/j.biortech.2012.03.008. Epub 2012 Mar 10.
Hydrolysis and acidification of grass silage (GS) was examined in leaching bed reactors (LBRs) under organic loading rates (OLRs) of 0.5, 0.8 and 1.0 kg volatile solids (VS)/m(3)/day. The LBRs were run in duplicate over five consecutive batch tests (Batch tests 1-5) to examine the effects of pH, leachate dilution and addition of inoculum on the process of hydrolysis and acidification. The highest GS hydrolysis yields of 52-58%, acidification yields of 57-60% and VS removals of 62-66% were obtained in Batch test 4. Increasing OLRs affected the hydrolysis yield negatively. In Batch test 4, the reduction of lignocellulosic materials was up to 74.4% of hemicellulose, 30.1% of cellulose and 9.3% of lignin within 32 days. Cellulase activity can be used as an indicator for the hydrolysis process. Methane production from the LBRs only accounted for 10.0-13.8% of the biological methane potential of GS.
采用渗滤床反应器（LBR）研究了在 0.5、0.8 和 1.0 kg 挥发性固体（VS）/m(3)/d 有机负荷率（OLR）条件下青贮牧草（GS）的水解酸化过程。LBR 在五个连续批次试验（批次试验 1-5）中重复运行，以考察 pH 值、渗滤液稀释和接种物添加对水解酸化过程的影响。在批次试验 4 中，GS 的水解产率最高可达 52-58%，酸化产率为 57-60%，VS 去除率为 62-66%。OLR 的增加对水解产率有负面影响。在批次试验 4 中，木质纤维素材料的减少量达到了半纤维素的 74.4%、纤维素的 30.1%和木质素的 9.3%，在 32 天内。纤维素酶活性可作为水解过程的指标。LBR 产生的甲烷仅占 GS 生物甲烷潜力的 10.0-13.8%。
