Department of Biological and Environmental Science, University of Jyväskylä, Finland.
FEMS Microbiol Ecol. 2010 Jun;72(3):496-506. doi: 10.1111/j.1574-6941.2010.00850.x. Epub 2010 Feb 16.
Six batch leach bed (LB) reactors, installed in parallel and connected to a common upflow anaerobic sludge blanket reactor, were fed with grass silage and operated at 35 (+/-1) degrees C. The development and distribution of microorganisms, which firmly and loosely attached to solid materials, and presented in the leachate in the LB reactors, were investigated by 16S rRNA gene-based terminal restriction fragment length polymorphism and clone library analyses. The phylotypes and their relative abundance changed in the respective bacterial community throughout the 49-day run and showed differences between the communities. Large numbers of phylotypes were detected from day 10 onwards. On day 17, the majority of phylotypes in the bacterial community firmly attached to solid residues affiliated to the classes Clostridia and Bacteroidetes. There were high numbers of the phylotypes in the leachate bacterial community. They were closely related to members of classes Clostridia, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, and OP10. The Clostridium-like species clearly dominated the bacterial community. Archaea were only found in the solid residues on day 17 and in the leachate on days 10 and 17. The majority of the Archaea fell within the hydrogenotrophic genus Methanobacterium. The organism assigned to the aceticlastic genus Methanosarcina was only present in the solid residues.
六个批次的渗滤床（LB）反应器平行安装，并与一个共同的上流厌氧污泥毯反应器相连，用草青贮饲料进行进料，并在 35（+/-1）摄氏度下运行。通过 16S rRNA 基因末端限制性片段长度多态性和克隆文库分析，研究了微生物的发展和分布，这些微生物牢固地和松散地附着在固体物质上，并存在于 LB 反应器中的渗滤液中。在 49 天的运行过程中，各细菌群落中的菌群和相对丰度发生了变化，并表现出群落之间的差异。从第 10 天开始，大量的菌群被检测到。第 17 天，牢固附着在固体残渣上的细菌群落中的大多数菌群属于厚壁菌门和拟杆菌门。在渗滤液细菌群落中有大量的菌群。它们与厚壁菌门、拟杆菌门、β变形菌门、α变形菌门、γ变形菌门和 OP10 的成员密切相关。类似于梭菌的物种明显主导了细菌群落。古菌仅在第 17 天的固体残渣和第 10 天和第 17 天的渗滤液中发现。大多数古菌属于产氢甲烷杆菌属。仅在固体残渣中存在归属于乙酸营养型甲烷菌属的生物体。
