Gobeev V N, Berezov T T
Vopr Med Khim. 1979 Jul-Aug;25(4):480-4.
Activities of hexokinase, glucokinase, cytochrome oxidase as well as amount of mitochondrial protein and subcellular distribution of hexokinase were studied in rat liver tissue after administration of acetyl aminofluorene and diethyl nitrosamine. Activity of the enzymes was altered in the same direction both in the primary induced hepatomas and in transplantable tumors of liver tissue. Glucokinase was not found but the fraction of hexokinase bound to mitochondrial membranes was observed in all the primary hepatomas studied; in this property the tumors resembled the embryonal liver tissue, various tissues of mature animals and transplantable hepatomas. This pattern of distribution of the enzymes reflects biochemical and functional disdifferentiation of the hepatomas. Properties of the bound hexokinase from the hepatoma were similar to those of the enzyme from embryonal liver tissue and, hence, they were distinct as compared with the enzymatic properties of hexokinase in the transplantable hepatomas.
在给予乙酰氨基芴和二乙基亚硝胺后，对大鼠肝组织中的己糖激酶、葡萄糖激酶、细胞色素氧化酶活性以及线粒体蛋白含量和己糖激酶的亚细胞分布进行了研究。在原发性诱导肝癌和肝组织移植瘤中，酶的活性均朝着相同方向改变。未发现葡萄糖激酶，但在所研究的所有原发性肝癌中均观察到与线粒体膜结合的己糖激酶部分；在这一特性上，肿瘤类似于胚胎肝组织、成熟动物的各种组织以及可移植肝癌。酶的这种分布模式反映了肝癌的生化和功能去分化。肝癌中结合型己糖激酶的特性与胚胎肝组织中的酶相似，因此，与可移植肝癌中己糖激酶的酶学特性相比，它们是不同的。
