Sözen Selim, Topuz Omer, Uzun Abdurrahman Selçuk, Cetinkünar Süleyman, Das Koray
Adana Numune Training and Research Hospital, General Surgery, Adana, Turkey.
Ann Ital Chir. 2012 Mar-Apr;83(2):143-8.
Ischemia/reperfusion (IR) injury of the intestine is a major problem in abdominal pathological condition and is associated with a high morbidity and mortality. The purpose of the study is to determine whether glutamine and melatonin can prevent BT of small intestinal IR injury in rats.
Forty Wistar-albino rats with a weight of 200 to 250 g were used in the study. They were randomly divided into four groups (n=10 for each group): sham operated group (Group I), IR group (Group II), IR+ glutamine treatment group (Group III) and IR+ melatonin treatment group (Group IV). All animals were given 10(10) E. Coli by orogastric intubation 12 hours before sampling. Seventy-two hours after the first operation, mesenteric lymph node and blood samples were obtained and cultured Two cc blood samples were obtained for a Polymerase chain reaction study. A piece of terminal ileum was also sampled for histopathologic examination.
Mesenteric lymph node and blood cultures of all control animals were positive for microbiological growth, and polymerase chain reaction results were positive in seven of the eight rats. Histopathologically, edema, vasodilatation and inflammatory cell infiltration were found to be less in the other groups in comparison to the control group. The incidence of bacterial translocation was decreased in all treatment groups as compared to the control group.
Glutamine and Melatonin reduced the incidence of BT in intestinal I/R. rats. These results suggest that glutamine and melatonin would be clinically useful in the treatment of intestinal I/R injury.
肠道缺血/再灌注（IR）损伤是腹部病理状况中的一个主要问题，与高发病率和死亡率相关。本研究的目的是确定谷氨酰胺和褪黑素是否能预防大鼠小肠IR损伤的细菌易位（BT）。
本研究使用了40只体重为200至250克的Wistar白化大鼠。它们被随机分为四组（每组n = 10）：假手术组（I组）、IR组（II组）、IR +谷氨酰胺治疗组（III组）和IR +褪黑素治疗组（IV组）。在取样前12小时，所有动物经口胃插管给予10（10）大肠杆菌。首次手术后72小时，获取肠系膜淋巴结和血样并进行培养。采集2毫升血样用于聚合酶链反应研究。还取一段回肠末端进行组织病理学检查。
所有对照动物的肠系膜淋巴结和血培养微生物生长均为阳性，8只大鼠中有7只聚合酶链反应结果为阳性。组织病理学上，与对照组相比，其他组的水肿、血管扩张和炎性细胞浸润较少。与对照组相比，所有治疗组的细菌易位发生率均降低。
谷氨酰胺和褪黑素降低了肠道I/R大鼠的BT发生率。这些结果表明，谷氨酰胺和褪黑素在治疗肠道I/R损伤方面具有临床应用价值。
