Rosero Olivér, Ónody Péter, Kovács Tibor, Molnár Dávid, Lotz Gábor, Tóth Szilárd, Turóczi Zsolt, Fülöp András, Garbaisz Dávid, Harsányi László, Szijártó Attila
1st Department of Surgery, Semmelweis University, Ulloi Street 78, Budapest 1082, Hungary.
Department of Human Morphology and Developmental Biology, Semmelweis University, Tuzolto Street 58, Budapest 1094, Hungary.
Biomed Res Int. 2014;2014:210901. doi: 10.1155/2014/210901. Epub 2014 May 14.
Mesenteric ischemia-reperfusion (IR) is associated with impairment of the gut barrier function and the initiation of a proinflammatory cascade with life-threatening results. Therefore methods directed to ameliorate IR injury are of great importance. We aimed at describing the effects of postconditioning (PC) on the alterations of the intestinal mucosal function and the inflammatory response upon mesenteric IR.
Male Wistar rats were gavaged with green fluorescent protein-expressing E. coli suspensions. Animals were randomized into three groups (n = 15), sham-operated, IR-, and PC-groups, and underwent 60 minutes of superior mesenteric artery occlusion, followed by 6 hours of reperfusion. Postconditioning was performed at the onset of reperfusion. Blood and tissue samples were taken at the end of reperfusion, for histological, bacteriological, and plasma examinations.
The PC-group presented a more favorable claudin-2, claudin-3, claudin-4, and zonula occludens-1 membrane expression profile, and significantly lower rates of bacterial translocation to distant organs and plasma D-lactate levels compared to the IR-group. Histopathological lesions, plasma I-FABP, IL-6, and TNF- α levels were significantly lower in the PC-group compared to the IR-group.
The use of postconditioning improved the integrity of the intestinal mucosal barrier upon mesenteric IR, and thus reduced the incidence of bacterial translocation and development of a systemic inflammatory response.
肠系膜缺血再灌注（IR）与肠道屏障功能受损以及引发具有生命威胁后果的促炎级联反应有关。因此，旨在改善IR损伤的方法非常重要。我们旨在描述后处理（PC）对肠系膜IR后肠黏膜功能改变和炎症反应的影响。
给雄性Wistar大鼠灌胃表达绿色荧光蛋白的大肠杆菌悬液。将动物随机分为三组（n = 15），即假手术组、IR组和PC组，进行60分钟的肠系膜上动脉闭塞，随后再灌注6小时。在再灌注开始时进行后处理。在再灌注结束时采集血液和组织样本，用于组织学、细菌学和血浆检查。
与IR组相比，PC组呈现出更有利的紧密连接蛋白-2、紧密连接蛋白-3、紧密连接蛋白-4和闭合蛋白-1膜表达谱，细菌向远处器官移位的发生率和血浆D-乳酸水平显著更低。与IR组相比，PC组的组织病理学损伤、血浆I-FABP、IL-6和TNF-α水平显著更低。
后处理的应用改善了肠系膜IR后肠黏膜屏障的完整性，从而降低了细菌移位的发生率和全身炎症反应的发生。
