Faculty of Engineering and Science, Sogn og Fjordane University College, Sogndal, Norway.
J Fish Dis. 2012 Jun;35(6):455-60. doi: 10.1111/j.1365-2761.2012.01355.x. Epub 2012 Mar 29.
The general structure and cell types in kidney and spleen in Buenos Aires tetra, Hyphessobrycon anisitsi, family Characidae, are described. The capability and capacity of these organs to clean foreign ferritin from the blood stream are analysed and compared. Head kidney was mainly composed of neutrophils, macrophages, lymphocytes and other white blood cells, whereas unmatured and matured red blood cells were few in number. Spleen often contained much red pulp, that is mainly matured red blood cells between splenic cords, often with some macrophages and neutrophils in the latter. Occasionally, this pulp contained large volumes of unmatured red blood cells, particularly in the periphery of the spleen. The splenic white pulp consisted of ellipsoids composed of an inner endothelial layer covered by a thick sheet of white blood cells, which in the periphery consisted mainly of macrophages. Erythrocytes occupied nearly the entire splenic volume in some specimens, whereas up to half of this volume was filled by ellipsoid macrophages, neutrophils, lymphocytes and other white blood cells in other specimens. The macrophages and sinusoidal endothelial cells in kidney and spleen from ferritin-injected specimens were tightly packed by yellow-brown granules or Prussian blue precipitations, in tissue treated with Mallory stain or acid ferrocyanide, respectively, suggesting a large uptake of foreign ferritin. In the present tetra large amounts of white blood cells are developed in head kidney, where macrophages and sinusoidal endothelial cells play important roles in the cleansing of scavenger and foreign molecules and particles from the blood stream. The spleen seems primarily to be a site for iron recycling and production and storage of red blood cells. Sometimes, however, it was rich in macrophages, neutrophils, lymphocytes and other white blood cells, suggesting functions like blood cleansing and non-specific and specific defence in such specimens.
描述了布宜诺斯艾利斯四齿脂鲤（Hyphessobrycon anisitsi）的肾脏和脾脏的一般结构和细胞类型。分析并比较了这些器官从血液中清除外来铁蛋白的能力和容量。头肾主要由中性粒细胞、巨噬细胞、淋巴细胞和其他白细胞组成，而未成熟和成熟的红细胞数量较少。脾脏通常含有大量红髓，即脾索之间的主要成熟红细胞，后者常有一些巨噬细胞和中性粒细胞。偶尔，这种髓质含有大量未成熟的红细胞，特别是在脾脏的外围。脾脏的白髓由椭圆形组成，由内皮细胞内层和厚厚的白细胞层组成，在周围主要由巨噬细胞组成。在一些标本中，红细胞几乎占据了脾脏的全部体积，而在其他标本中，一半以上的脾脏体积被椭圆形巨噬细胞、中性粒细胞、淋巴细胞和其他白细胞填充。用 Mallory 染色或酸亚铁氰化物处理组织后，肾脏和脾脏中注射铁蛋白的标本中的巨噬细胞和窦内皮细胞被黄棕色颗粒或普鲁士蓝沉淀紧密包裹，提示大量摄取了外来铁蛋白。在本四齿脂鲤中，大量的白细胞在头肾中发育，其中巨噬细胞和窦内皮细胞在清除血液中的吞噬和外来分子和颗粒方面发挥着重要作用。脾脏似乎主要是铁回收和红细胞生成、储存的场所。然而，有时它富含巨噬细胞、中性粒细胞、淋巴细胞和其他白细胞，这表明在这些标本中具有血液净化和非特异性和特异性防御等功能。
