Appell R A, Evans P R
Fertil Steril. 1977 Dec;28(12):1329-32. doi: 10.1016/s0015-0282(16)42978-x.
Semen specimens from fertile prevasectomy patients maintained at 4 degrees, 20 degrees, and 37 degrees C were evaluated at 3, 6, 12, and 18 hours after collection. Sperm viability, assessed by eosin-nigrosin stain, and motility decreased with time at 20 degrees and 37 degrees C, but at a significantly higher rate at 37 degrees C (where the motility was halved by 12 hours). The slope of the decrease in viability closely paralleled that of the motility except at 4 degrees C, where motility was nearly absent at 6 hours but viability was retained through 18 hours. Bacterial counts rose markedly and the pH fell at 37 degrees C, which may explain the decrease in motility and viability. It is clear from this study that semen should be kept at room temperature (20 degrees C) and not at 37 degrees C if there is to be any delay in its analysis, or a falsely lowered motility will result.
对输精管切除术前生育能力正常的患者采集的精液标本，分别在4℃、20℃和37℃保存，并在采集后3、6、12和18小时进行评估。通过伊红-黑色素染色评估的精子活力和运动能力在20℃和37℃下随时间下降，但在37℃下降速度明显更快（12小时时运动能力减半）。活力下降的斜率与运动能力下降的斜率密切平行，4℃时除外，4℃时6小时几乎没有运动能力，但18小时仍保留活力。37℃时细菌计数显著上升，pH值下降，这可能解释了运动能力和活力的下降。从这项研究可以清楚地看出，如果精液分析有任何延迟，应将其保存在室温（20℃）而不是37℃，否则会导致运动能力错误降低。
