Appell R A, Evans P R
Fertil Steril. 1978 Oct;30(4):436-8. doi: 10.1016/s0015-0282(16)43578-8.
The previous demonstration that sperm kept at body temperature (37 degrees C) had a marked deterioration in motility accompanied by an overgrowth of bacteria in the semen and a concomitant decrease in pH led to this study to test the hypothesis that the decrease in motility was caused by the bacteria or by bacterial alteration of seminal pH. Semen specimens from fertile prevasectomy patients with and without added antibiotics were maintained at 20 degrees C and 37 degrees C and evaluated at 3, 12, and 18 hours after collection. There was still a significant deterioration in spermatozoal motility in the samples kept at 37 degrees C even when bacterial growth and change in pH were prevented by buffered antibiotics. Although the decrease in spermatozoal motility at body temperature may in part be attributed to bacterial growth or the products of bacterial metabolism, clearly another factor is present related to time and temperature and independent of the presence of bacteria.
先前的研究表明，保存在体温（37摄氏度）下的精子活力显著下降，同时精液中细菌过度生长，pH值随之降低。由此开展了本研究，以验证精子活力下降是由细菌还是由细菌导致的精液pH值改变所引起这一假设。对输精管切除术前生育能力正常的患者的精液样本，添加或不添加抗生素后分别保存在20摄氏度和37摄氏度，并在采集后3小时、12小时和18小时进行评估。即使使用缓冲抗生素防止了细菌生长和pH值变化，保存在37摄氏度的样本中精子活力仍显著下降。尽管体温下精子活力下降可能部分归因于细菌生长或细菌代谢产物，但显然还存在另一个与时间和温度相关且独立于细菌存在的因素。
