School of Biosciences & Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Biochem Biophys Res Commun. 2012 Apr 20;420(4):834-8. doi: 10.1016/j.bbrc.2012.03.083. Epub 2012 Mar 23.
Xanthorrhizol is a plant-derived pharmacologically active sesquiterpenoid compound isolated from Curcuma xanthorrhiza. Previously, we have reported that xanthorrhizol inhibited the proliferation of HepG2 human hepatoma cells by inducing apoptotic cell death via caspase activation. Here, we attempt to further elucidate the mode of action of xanthorrhizol. Apoptosis in xanthorrhizol-treated HepG2 cells as observed by scanning electron microscopy was accompanied by truncation of BID; reduction of both anti-apoptotic Bcl-2 and Bcl-X(L) expression; cleavage of PARP and DFF45/ICAD proteins and DNA fragmentation. Taken together, these results suggest xanthorrhizol as a potent antiproliferative agent on HepG2 cells by inducing apoptosis via Bcl-2 family members. Hence we proposed that xanthorrhizol could be used as an anti-liver cancer drug for future studies.
姜黄素是一种从姜黄中分离得到的植物来源的具有药理活性的倍半萜化合物。先前，我们已经报道姜黄素通过激活半胱天冬酶诱导细胞凋亡而抑制 HepG2 人肝癌细胞的增殖。在这里，我们试图进一步阐明姜黄素的作用模式。扫描电子显微镜观察到姜黄素处理的 HepG2 细胞中的细胞凋亡伴随着 BID 的截断；抗凋亡 Bcl-2 和 Bcl-X(L)表达减少；PARP 和 DFF45/ICAD 蛋白的裂解和 DNA 片段化。综上所述，这些结果表明姜黄素通过 Bcl-2 家族成员诱导细胞凋亡对 HepG2 细胞具有很强的增殖抑制作用。因此，我们提出姜黄素可作为未来研究的肝癌治疗药物。
