Kariyone A, Tanabe M, Juji T, Kano K, Takiguchi M
Department of Immunology, University of Tokyo, Japan.
J Immunol. 1990 Dec 1;145(11):3714-8.
The surface expression of two HLA-C blank Ag (Cb-1 and Cb-2) on PBL was investigated with Cb-1- and Cb-2-specific CTL clones generated by the stimulation of the HLA-C blank Ag on transfected Hmy2CIR cells. The Cb-1- and Cb-2-specific CTL clones could lyse EBV-transformed B cells and PHA-induced T cells from which the HLA-C blank genes were derived. Furthermore, the reactivity of these CTL clones with PHA-induced T cells was blocked by HLA class I monomorphic mAb. These results demonstrated that the HLA-C blank Ag are expressed on the surfaces of PBL. Thus, despite the fact that the HLA-C blank Ag are expressed on normal PBL, they are incapable of generating corresponding alloantibodies. On the other hand, the present study demonstrated that these Ag on normal PBL are able to induce specific CTL and that the capacity of these Ag to induce allogeneic CTL is almost identical to that of HLA-B Ag, indicating that they may function as alloantigens in vivo and play a significant role in the rejection of organ grafts and in the graft-versus-host reaction in bone marrow transplantation.
利用转染的Hmy2CIR细胞上的HLA - C空白抗原刺激产生的Cb - 1和Cb - 2特异性CTL克隆，研究了PBL上两种HLA - C空白抗原（Cb - 1和Cb - 2）的表面表达。Cb - 1和Cb - 2特异性CTL克隆能够裂解来自HLA - C空白基因的EBV转化的B细胞和PHA诱导的T细胞。此外，这些CTL克隆与PHA诱导的T细胞的反应性被HLA I类单态性单克隆抗体阻断。这些结果表明HLA - C空白抗原在PBL表面表达。因此，尽管HLA - C空白抗原在正常PBL上表达，但它们不能产生相应的同种抗体。另一方面，本研究表明正常PBL上的这些抗原能够诱导特异性CTL，并且这些抗原诱导同种异体CTL的能力与HLA - B抗原几乎相同，表明它们可能在体内作为同种抗原发挥作用，并在器官移植排斥反应和骨髓移植的移植物抗宿主反应中起重要作用。
