Yannelli J R, Moore L C, Engelhard V H
J Immunol. 1985 Aug;135(2):900-5.
Eleven cytotoxic T lymphocyte (CTL) clones were derived from C57BL/6 spleen cells immunized with HLA-B7 expressing human lymphoblastoid cell lines. Reactivity against HLA-B7 was initially established because the clones lysed 2 target cells that shared only HLA-B7 with the immunizing cell line and they did not lyse five other cell lines that were HLA-B7 negative but expressed other class I or class II antigens found on the immunizing cell. Six of the clones were subsequently shown to lyse all tested HLA-B7-positive B and T lymphoid cell lines, peripheral blood lymphocytes, and a murine L cell that expressed HLA-B7 as a consequence of DNA-mediated gene transfer. On the basis of the inability of the clones to lyse a panel of HLA-B7-negative cell lines, up to 18 other class I antigens could be eliminated as being cross-reactively recognized. However, two of the clones recognized a single HLA-B7-negative cell line. It is suggested that in these cases the clones were cross-reactively recognizing the HLA-B27 or HLA-B40 antigens that were present on these target cells. The remaining five CTL clones failed to lyse one out of seven tested HLA-B7-positive lymphoid lines (either RPMI-1788 or DR1B) and failed to lyse peripheral blood lymphocytes from one out of three tested HLA-B7-positive individuals. These five clones also did not recognize the HLA-B7-positive murine L cell. However, based on analysis with a large target cell panel, the reactivity pattern of these five clones could only be correlated with recognition of HLA-B7. This conclusion is further supported by antibody-blocking studies to be reported elsewhere. As before, lysis of single HLA-B7-negative target cells by two of the clones could be ascribed to recognition of HLA-B27 or HLA-B40. The results show that murine clones raised against HLA-B7 exhibit a high degree of specificity for determinants that are unique or largely confined to the HLA-B7 alloantigen. In addition, these clones define different antigenic determinants on the molecule. Thus, such clones appear to be excellent candidates for use as human tissue typing reagent. The results further show that there is a strong correlation between recognition of particular HLA-B7-positive human cell lines and recognition of the HLA-B7 expressing murine L cell. Possible reasons for such a correlation and their relationship to the general phenomenon of CTL recognition are discussed.
从用表达HLA - B7的人淋巴母细胞系免疫的C57BL/6脾细胞中获得了11个细胞毒性T淋巴细胞（CTL）克隆。最初确定了针对HLA - B7的反应性，因为这些克隆能够裂解2个仅与免疫细胞系共享HLA - B7的靶细胞，而它们不能裂解另外5个HLA - B7阴性但表达免疫细胞上发现的其他I类或II类抗原的细胞系。随后发现其中6个克隆能够裂解所有测试的HLA - B7阳性B和T淋巴细胞系、外周血淋巴细胞以及由于DNA介导的基因转移而表达HLA - B7的小鼠L细胞。基于这些克隆无法裂解一组HLA - B7阴性细胞系，多达18种其他I类抗原可被排除为交叉反应识别的对象。然而，其中2个克隆识别了一个单一的HLA - B7阴性细胞系。推测在这些情况下，克隆是交叉反应识别这些靶细胞上存在的HLA - B27或HLA - B40抗原。其余5个CTL克隆未能裂解7个测试的HLA - B7阳性淋巴细胞系中的1个（RPMI - 1788或DR1B），也未能裂解3个测试的HLA - B7阳性个体中的1个的外周血淋巴细胞。这5个克隆也不识别HLA - B7阳性的小鼠L细胞。然而，基于对大量靶细胞的分析，这5个克隆的反应模式只能与对HLA - B7的识别相关。抗体阻断研究（将在其他地方报道）进一步支持了这一结论。如前所述，其中2个克隆对单个HLA - B7阴性靶细胞的裂解可归因于对HLA - B27或HLA - B40的识别。结果表明，针对HLA - B7产生的小鼠克隆对独特的或主要局限于HLA - B7同种异体抗原的决定簇表现出高度特异性。此外，这些克隆定义了该分子上不同的抗原决定簇。因此，此类克隆似乎是用作人类组织分型试剂的极佳候选者。结果还表明，对特定HLA - B7阳性人类细胞系的识别与对表达HLA - B7的小鼠L细胞的识别之间存在很强的相关性。讨论了这种相关性的可能原因及其与CTL识别一般现象的关系。
