Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Carver College of Medicine, Iowa City, IA, USA.
Vaccine. 2012 May 21;30(24):3646-52. doi: 10.1016/j.vaccine.2012.03.038. Epub 2012 Mar 29.
The Ig levels and antibody repertoire diversification in fetal piglets infected with an attenuated Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were measured. Serum Ig levels were greatly elevated in PRRSV-infected fetuses; IgG was elevated >50-fold, IgM>5-15-fold and IgA>2-fold compared to control fetuses. Their IgM to IgG to IgA profile was the same as that in isolator piglets infected for the same period with wild-type PRRSV. Fetal animals showed less repertoire diversification than even isolator piglets that were maintained germfree (GF) while the repertoire diversification index (RDI) for PRRSV-infected isolator piglets was 10-fold higher and comparable to littermates infected with swine influenza (S-FLU). However, when expressed as the RDI:Ig ratio, infected fetuses appeared 10-fold less capable of repertoire diversification than uninfected littermates and GF isolator piglets. Compared to S-FLU isolator piglets that resolve the infection, the RDI:Ig of PRRSV-infected isolator piglets was 100-fold lower. Overall, infection of fetuses with an attenuated virus shows the same immune dysregulation seen postnatally in wild type infected isolator piglets, indicating that: (a) attenuation did not alter the ability of the virus to cause dysregulation and (b) the isolator infectious model reflects the fetal disease.
检测了感染弱毒猪繁殖与呼吸综合征病毒（PRRSV）的仔猪的 Ig 水平和抗体库多样性。PRRSV 感染胎儿的血清 Ig 水平显著升高；与对照胎儿相比，IgG 升高了>50 倍，IgM 升高了 5-15 倍，IgA 升高了 2 倍。它们的 IgM 对 IgG 对 IgA 谱与用野生型 PRRSV 感染相同时间的隔离器仔猪相同。与即使在无菌（GF）条件下维持的隔离器仔猪相比，胎儿动物的多样性较少，但感染 PRRSV 的隔离器仔猪的多样性指数（RDI）高 10 倍，与感染猪流感（S-FLU）的同窝仔猪相当。然而，当以 RDI:Ig 比值表示时，感染胎儿的多样性能力比未感染的同窝仔猪和 GF 隔离器仔猪低 10 倍。与解决感染的 S-FLU 隔离器仔猪相比，感染 PRRSV 的隔离器仔猪的 RDI:Ig 低 100 倍。总的来说，感染弱毒的胎儿表现出与感染野生型隔离器仔猪在出生后相同的免疫失调，表明：（a）减毒并未改变病毒引起失调的能力，（b）隔离器传染性模型反映了胎儿疾病。
