一些嘧啶化合物对大鼠脑单胺氧化酶-B 的体外影响。
Effect of some pyrimidine compounds on rat brain monoamine oxidase-B in vitro.
机构信息
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
出版信息
J Physiol Biochem. 2012 Dec;68(4):475-84. doi: 10.1007/s13105-012-0160-4. Epub 2012 Mar 31.
The effects of some pyrimidine compounds (PCs) including barbituric acid (BA) 5,5-diethyl barbituric acid (DEBA), 2-thiobarbituric acid (TBA), violuric acid (VA), 2-thiouracil (TU), and 6-amino-2-thiouracil (ATU) on the activity of rat brain monoamine oxidase-B (MAO-B) were investigated. The results revealed that MAO-B was activated by BA, DEBA, TBA, TU, and ATU, and the activation was structural, concentration, and time dependent. However, MAO-B was inhibited by VA in a noncompetitive and irreversible manner with an enzyme-inhibitor dissociation constant (K (i) value) of 32 nM and IC(50) equals to 19 nM. All the studied PCs changed both the optimum pH and temperature of MAO-B.
研究了几种嘧啶类化合物（PCs），包括巴比妥酸（BA）、5,5-二乙基巴比妥酸（DEBA）、2-硫代巴比妥酸（TBA）、尿刊酸（VA）、2-硫代尿嘧啶（TU）和6-氨基-2-硫代尿嘧啶（ATU）对大鼠脑单胺氧化酶-B（MAO-B）活性的影响。结果表明，BA、DEBA、TBA、TU 和 ATU 可激活 MAO-B，这种激活具有结构、浓度和时间依赖性。然而，VA 以非竞争性和不可逆的方式抑制 MAO-B，其酶抑制剂解离常数（K（i）值）为 32 nM，IC（50）值等于 19 nM。所有研究的 PCs 均改变了 MAO-B 的最适 pH 值和温度。
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