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使用碰撞诱导解离/电子转移解离质谱法分析糖肽。

Use of CID/ETD mass spectrometry to analyze glycopeptides.

作者信息

Mechref Yehia

机构信息

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas.

出版信息

Curr Protoc Protein Sci. 2012 Apr;Chapter 12:12.11.1-12.11.11. doi: 10.1002/0471140864.ps1211s68.

DOI:10.1002/0471140864.ps1211s68
PMID:22470127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3673024/
Abstract

Collision-induced dissociation (CID) tandem mass spectrometry (MS/MS) does not allow the characterization of glycopeptides because of the fragmentation of glycan structures and limited fragmentation of peptide backbones. Electron transfer dissociation (ETD) MS/MS, on the other hand, offers a complementary approach, prompting only peptide backbone fragmentation while keeping post-translational modifications intact. Characterization of glycopeptides using both CID and ETD is summarized in this unit. While CID provides information related to the composition of glycan moieties attached to a peptide backbone, ETD permits de novo sequencing of peptides. Radical anion transfer of electrons to the peptide backbone in ETD induces cleavage of the N-Cα bond. The glycan moiety is retained on the peptide backbone, largely unaffected by the ETD process, thus allowing the identification of the amino acid sequence of a glycopeptide and its glycosylation site. This unit discusses the use of both CID and ETD for better characterization of glycopeptides.

摘要

碰撞诱导解离（CID）串联质谱（MS/MS）无法对糖肽进行表征，因为聚糖结构会发生碎片化，且肽主链的碎片化程度有限。另一方面，电子转移解离（ETD）MS/MS提供了一种互补方法，仅促使肽主链发生碎片化，同时保持翻译后修饰完整。本单元总结了使用CID和ETD对糖肽进行表征的方法。虽然CID提供了与连接在肽主链上的聚糖部分组成相关的信息，但ETD允许对肽进行从头测序。在ETD中，电子向肽主链的自由基阴离子转移会诱导N-Cα键的断裂。聚糖部分保留在肽主链上，在很大程度上不受ETD过程的影响，从而能够鉴定糖肽的氨基酸序列及其糖基化位点。本单元讨论了使用CID和ETD来更好地表征糖肽。