Dzhagalov Ivan L, Melichar Heather J, Ross Jenny O, Herzmark Paul, Robey Ellen A
Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.
Curr Protoc Cytom. 2012 Apr;Chapter 12:Unit12.26. doi: 10.1002/0471142956.cy1226s60.
Two-photon microscopy is a powerful method for visualizing biological processes as they occur in their native environment in real time. The immune system uniquely benefits from this technology as most of its constituent cells are highly motile and interact extensively with each other and with the environment. Two-photon microscopy has provided many novel insights into the dynamics of the development and function of the immune system that could not have been deduced by other methods and has become an indispensible tool in the arsenal of immunologists. In this unit, we provide several protocols for preparation of various organs for imaging by two-photon microscopy that are intended to introduce the new user to some basic aspects of this method.
双光子显微镜是一种强大的方法，可实时可视化生物过程在其原生环境中的发生情况。免疫系统从这项技术中受益匪浅，因为其大多数组成细胞具有高度的运动性，并且彼此之间以及与环境广泛相互作用。双光子显微镜为免疫系统的发育和功能动力学提供了许多新颖的见解，这些见解是其他方法无法推导出来的，并且已成为免疫学家武器库中不可或缺的工具。在本单元中，我们提供了几种通过双光子显微镜对各种器官进行成像的制备方案，旨在向新用户介绍该方法的一些基本方面。
