为激光扫描荧光显微镜增添新维度。

Adding new dimensions to laser-scanning fluorescence microscopy.

作者信息

De A K, Goswami D

机构信息

Department of Chemistry, Indian Institute of Technology Kanpur, UP 208016, India.

出版信息

J Microsc. 2009 Feb;233(2):320-5. doi: 10.1111/j.1365-2818.2009.03122.x.

DOI:10.1111/j.1365-2818.2009.03122.x

PMID:19220698

Abstract

We describe a novel method of optical imaging by exploiting simple ideas borrowed from pulsed optics. We show that the use of ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy dramatically brings together several advantages offered by two widely used present day microscopic techniques, confocal and multi-photon fluorescence microscopy. The method appears as a novel tool in the context of laser-scanning fluorescence microscopy by having a 'built-in' 3D spatial resolution.

摘要

我们描述了一种利用从脉冲光学借鉴的简单理念进行光学成像的新方法。我们表明，在激光扫描荧光显微镜中使用超快脉冲单光子激发极大地融合了当今两种广泛使用的显微镜技术（共聚焦和多光子荧光显微镜）所提供的多种优势。通过具有“内置”的三维空间分辨率，该方法在激光扫描荧光显微镜领域呈现为一种新型工具。