Renal Transplant Unit, Department of Experimental Immunology, Academic Medical Center, Amsterdam, the Netherlands.
Clin Exp Immunol. 2012 May;168(2):241-50. doi: 10.1111/j.1365-2249.2011.04551.x.
Several assays to measure pre-existing allospecific T cell immunity in renal transplant candidates have been developed in the past years. In 46 patients, we used flow cytometry-based mixed lymphocyte culture to measure the precursor frequency and phenotype of alloreactive T cells before renal transplantation, using donor-specific or third-party cells for allostimulation. Allostimulation induced up-regulation of co-stimulatory molecules, chemokine receptors relevant for migration of T cells into the graft and effector proteins. Recipients prone for acute rejection had a higher precursor frequency of alloreactive CD8(+) T cells and a lower percentage of interleukin (IL)-7Rα expressing alloreactive CD8(+) T cells than non-rejectors. These data point to quantitative and qualitative differences between T cells of patients who will experience acute cellular rejection episodes from those who will not.
过去几年中，已经开发出了几种用于测量肾移植候选者中预先存在的同种异体 T 细胞免疫的检测方法。在 46 例患者中，我们使用基于流式细胞术的混合淋巴细胞培养，在肾移植前使用供体特异性或第三方细胞进行同种异体刺激，以测量同种反应性 T 细胞的前体频率和表型。同种异体刺激诱导共刺激分子、趋化因子受体的上调，这些受体与 T 细胞向移植物的迁移和效应蛋白有关。发生急性排斥反应的受者具有更高的同种反应性 CD8(+) T 细胞前体频率，而表达白细胞介素 (IL)-7Rα 的同种反应性 CD8(+) T 细胞的百分比低于非排斥者。这些数据表明，在经历急性细胞排斥反应的患者与不会经历急性细胞排斥反应的患者的 T 细胞之间存在定量和定性的差异。
