• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

分析 T 细胞同种异体抗原反应 供体特异性抗体的肾移植受者的直接途径。

Analysis of T-cell alloantigen response a direct pathway in kidney transplant recipients with donor-specific antibodies.

机构信息

Department of Urology, Hokkaido University Hospital, Sapporo, Japan.

Division of Renal Surgery and Transplantation, Jichi Medical University, Shimotsuke, Tochigi, Japan.

出版信息

Front Immunol. 2023 May 3;14:1164794. doi: 10.3389/fimmu.2023.1164794. eCollection 2023.

DOI:10.3389/fimmu.2023.1164794
PMID:37207202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10189043/
Abstract

Donor-specific antibodies (DSAs) are the main cause of graft loss over time. The direct pathway of alloantigen recognition is important in the pathogenesis of acute rejection. Recent studies have suggested that the direct pathway also contributes to the pathogenesis of chronic injury. Nevertheless, there are no reports on T-cell alloantigen response the direct pathway in kidney recipients with DSAs. We analyzed the T-cell alloantigen response the direct pathway in kidney recipients with DSAs (DSA+) or without DSAs (DSA-). A mixed lymphocyte reaction assay was implemented to assess the direct pathway response. DSA+ patients showed significantly higher CD8 and CD4 T cell responses to donor cells than DSA- patients. Furthermore, proliferating CD4 T cells showed a marked increase in Th1 and Th17 responses in DSA+ patients than in DSA- patients. In a comparison between anti-donor and third-party responses, the anti-donor CD8 and CD4 T cell response was significantly lower than the anti-third-party response. In contrast, the donor-specific hyporesponsiveness was absent in DSA+ patients. Our study demonstrated that DSA+ recipients have a greater potential for developing immune responses against the donor tissues the direct alloantigen recognition pathway. These data contribute to an understanding of DSAs pathogenicity during kidney transplantation.

摘要

供体特异性抗体（DSA）是导致移植物随时间丢失的主要原因。同种异体抗原识别的直接途径在急性排斥反应的发病机制中很重要。最近的研究表明，直接途径也有助于慢性损伤的发病机制。然而，目前尚无关于 DSA 肾移植受者中 T 细胞同种异体抗原反应直接途径的报道。我们分析了 DSA（DSA+）或无 DSA（DSA-）肾移植受者的 T 细胞同种异体抗原反应直接途径。实施混合淋巴细胞反应测定来评估直接途径反应。DSA+患者对供体细胞的 CD8 和 CD4 T 细胞反应明显高于 DSA-患者。此外，与 DSA-患者相比，增殖的 CD4 T 细胞在 DSA+患者中表现出 Th1 和 Th17 反应的明显增加。在与抗供体和第三方反应的比较中，抗供体 CD8 和 CD4 T 细胞反应明显低于抗第三方反应。相比之下，DSA+患者不存在供体特异性低反应性。我们的研究表明，DSA+受者对供体组织具有更强的免疫反应潜力，即直接同种抗原识别途径。这些数据有助于理解 DSAs 在肾移植过程中的致病性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/646503a28162/fimmu-14-1164794-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/a66e2e99c3d3/fimmu-14-1164794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/56cc484f1c7b/fimmu-14-1164794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/acd4d9d352c4/fimmu-14-1164794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/817027d71d31/fimmu-14-1164794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/8799a8ac48b1/fimmu-14-1164794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/05bea7852661/fimmu-14-1164794-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/646503a28162/fimmu-14-1164794-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/a66e2e99c3d3/fimmu-14-1164794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/56cc484f1c7b/fimmu-14-1164794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/acd4d9d352c4/fimmu-14-1164794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/817027d71d31/fimmu-14-1164794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/8799a8ac48b1/fimmu-14-1164794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/05bea7852661/fimmu-14-1164794-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d52/10189043/646503a28162/fimmu-14-1164794-g007.jpg

相似文献

1
Analysis of T-cell alloantigen response a direct pathway in kidney transplant recipients with donor-specific antibodies.分析 T 细胞同种异体抗原反应 供体特异性抗体的肾移植受者的直接途径。
Front Immunol. 2023 May 3;14:1164794. doi: 10.3389/fimmu.2023.1164794. eCollection 2023.
2
Clinical significance of low pre-transplant donor specific antibodies (DSA) in living donor kidney recipients with negative complement-dependent cytotoxicity crossmatches (CDCXM), and negative flow cytometry crossmatches (FLXM) - A single-center experience.移植前低供体特异性抗体（DSA）在阴性补体依赖性细胞毒性交叉匹配（CDCXM）和阴性流式细胞术交叉匹配（FLXM）的活体供肾受者中的临床意义 - 单中心经验。
Transpl Immunol. 2022 Oct;74:101672. doi: 10.1016/j.trim.2022.101672. Epub 2022 Jul 19.
3
Effect of C1q-binding donor-specific anti-HLA antibodies on the clinical outcomes of patients after renal transplantation: A systematic review and meta-analysis.补体 C1q 结合供体特异性抗 HLA 抗体对肾移植后患者临床结局的影响：系统评价和荟萃分析。
Transpl Immunol. 2022 Jun;72:101566. doi: 10.1016/j.trim.2022.101566. Epub 2022 Mar 4.
4
Comparative analysis of two methods to detect donor-specific anti-HLA antibodies after kidney transplant.肾移植后两种检测供者特异性抗人白细胞抗原抗体方法的比较分析
Transpl Immunol. 2018 Aug;49:7-11. doi: 10.1016/j.trim.2018.03.006. Epub 2018 Mar 22.
5
CD4+ T Cell Help Is Mandatory for Naive and Memory Donor-Specific Antibody Responses: Impact of Therapeutic Immunosuppression.CD4+ T 细胞辅助对于幼稚和记忆供体特异性抗体反应是必需的：治疗性免疫抑制的影响。
Front Immunol. 2018 Feb 19;9:275. doi: 10.3389/fimmu.2018.00275. eCollection 2018.
6
Adoptive transfusion of ex vivo donor alloantigen-stimulated CD4(+)CD25(+) regulatory T cells ameliorates rejection of DA-to-Lewis rat liver transplantation.体外供体同种异体抗原刺激的CD4(+)CD25(+)调节性T细胞的过继性输血可改善DA到Lewis大鼠肝移植的排斥反应。
Surgery. 2007 Jul;142(1):67-73. doi: 10.1016/j.surg.2007.02.014.
7
Virus-Specific CD8(+) T Cells Cross-Reactive to Donor-Alloantigen Are Transiently Present in the Circulation of Kidney Transplant Recipients Infected With CMV and/or EBV.病毒特异性 CD8(+) T 细胞对供体同种异体抗原具有交叉反应性，在感染 CMV 和/或 EBV 的肾移植受者的循环中短暂存在。
Am J Transplant. 2016 May;16(5):1480-91. doi: 10.1111/ajt.13618. Epub 2016 Jan 25.
8
Pretransplant immunologic risk assessment of kidney transplant recipients with donor-specific anti-human leukocyte antigen antibodies.移植前具有供体特异性抗人类白细胞抗原抗体的肾移植受者的免疫风险评估。
Transplantation. 2014 Nov 27;98(10):1082-8. doi: 10.1097/TP.0000000000000191.
9
The kinetics of tolerance induction by nondepleting anti-CD4 monoclonal antibody (RIB 5/2) plus intravenous donor alloantigen administration.非清除性抗CD4单克隆抗体（RIB 5/2）联合静脉注射供体同种异体抗原诱导耐受的动力学
Transplantation. 2000 Jan 27;69(2):285-93. doi: 10.1097/00007890-200001270-00015.
10
Lack of Association between Pretransplant Donor-Specific Antibodies and Posttransplant Kidney Outcomes in Simultaneous Liver-Kidney Transplant Recipients with Rabbit Anti-Thymocyte Globulin Induction and Steroid-Free Protocol.在接受兔抗胸腺细胞球蛋白诱导和无类固醇方案的同时肝肾移植受者中，移植前供体特异性抗体与移植后肾脏结局之间缺乏关联。
Nephron. 2020;144(3):126-137. doi: 10.1159/000505460. Epub 2020 Jan 31.

引用本文的文献

1
Cross-organ hierarchy of HLA molecular mismatches in donor-specific antibody development in solid organ transplantations.实体器官移植中供者特异性抗体产生过程中HLA分子错配的跨器官层级关系
Cell Rep Med. 2025 Jun 17;6(6):102153. doi: 10.1016/j.xcrm.2025.102153. Epub 2025 May 30.
2
Pretransplant, Th17 dominant alloreactivity in highly sensitized kidney transplant candidates.移植前，高度致敏的肾移植受者中Th17主导的同种异体反应性。
Front Transplant. 2024 Apr 8;3:1336563. doi: 10.3389/frtra.2024.1336563. eCollection 2024.
3
Analysis of IL-17A, IL-17F, and miR-146a-5p Prior to Transplantation and Their Role in Kidney Transplant Recipients.

本文引用的文献

1
T cell antigenicity and immunogenicity of allogeneic exosomes.同种异体外泌体的 T 细胞抗原性和免疫原性。
Am J Transplant. 2021 Jul;21(7):2583-2589. doi: 10.1111/ajt.16591. Epub 2021 Apr 30.
2
Subclinical Antibody-mediated Rejection After Kidney Transplantation: Treatment Outcomes.移植肾后亚临床抗体介导的排斥反应：治疗结局。
Transplantation. 2019 Aug;103(8):1722-1729. doi: 10.1097/TP.0000000000002566.
3
Donor-specific hypo-responsiveness occurs in simultaneous liver-kidney transplant recipients after the first year.供者特异性低反应性在肝-肾联合移植受者中于第 1 年后出现。
移植前白细胞介素-17A、白细胞介素-17F和微小RNA-146a-5p的分析及其在肾移植受者中的作用
J Clin Med. 2024 May 15;13(10):2920. doi: 10.3390/jcm13102920.
Kidney Int. 2018 Jun;93(6):1465-1474. doi: 10.1016/j.kint.2018.01.022. Epub 2018 Apr 12.
4
Graft dysfunction in chronic antibody-mediated rejection correlates with B-cell-dependent indirect antidonor alloresponses and autocrine regulation of interferon-γ production by Th1 cells.慢性抗体介导性排斥反应中的移植物功能障碍与B细胞依赖性间接抗供体同种异体反应以及Th1细胞对干扰素-γ产生的自分泌调节相关。
Kidney Int. 2017 Feb;91(2):477-492. doi: 10.1016/j.kint.2016.10.009. Epub 2016 Dec 15.
5
Donor exosomes rather than passenger leukocytes initiate alloreactive T cell responses after transplantation.移植后，引发同种异体反应性T细胞反应的是供体来源的外泌体而非过客白细胞。
Sci Immunol. 2016 Jul 14;1(1). doi: 10.1126/sciimmunol.aaf8759.
6
Induced regulatory T cells in allograft tolerance via transient mixed chimerism.通过短暂混合嵌合实现同种异体移植耐受中的诱导调节性T细胞。
JCI Insight. 2016 Jul 7;1(10). doi: 10.1172/jci.insight.86419.
7
Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction.慢性移植肾失功的肾移植受者中Th17细胞表型增加。
PLoS One. 2015 Dec 30;10(12):e0145258. doi: 10.1371/journal.pone.0145258. eCollection 2015.
8
The imbalance between Tregs, Th17 cells and inflammatory cytokines among renal transplant recipients.肾移植受者中调节性T细胞、辅助性T细胞17和炎性细胞因子之间的失衡。
BMC Immunol. 2015 Sep 23;16:56. doi: 10.1186/s12865-015-0118-8.
9
Comment on "Tracking donor-reactive T cells: Evidence for clonal deletion in tolerant kidney transplant patients".关于“追踪供体反应性T细胞：肾移植耐受患者克隆清除的证据”的评论
Sci Transl Med. 2015 Jul 22;7(297):297le1. doi: 10.1126/scitranslmed.aab1994.
10
B-lymphocytes support and regulate indirect T-cell alloreactivity in individual patients with chronic antibody-mediated rejection.B 淋巴细胞在个体慢性抗体介导的排斥反应患者中支持和调节间接 T 细胞同种异体反应。
Kidney Int. 2015 Sep;88(3):560-8. doi: 10.1038/ki.2015.100. Epub 2015 Apr 1.