Budda Sirintip, Butryee Chaniphun, Tuntipopipat Siriporn, Rungsipipat Anudep, Wangnaithum Supradit, Lee Jeong-Sang, Kupradinun Piengchai
Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand.
Asian Pac J Cancer Prev. 2011;12(12):3221-8.
Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisine and has some medicinal properties. Previous studies have shown potentially antioxidant, antitumor promoter, anticlastogen and anticarcinogen activities both in vitro and in vivo. The present study was conducted to investigate chemopreventive effects on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted colon carcinogenesis in mice. Male ICR mice were divided into 8 groups: Group 1 served as a negative control; Group 2 received AOM/DSS as a positive control; Groups 3-5 were fed boiled freeze-dried M. oleifera (bMO) at 1.5%, 3.0% and 6.0%, respectively supplemented in basal diets for 5 weeks; Groups 6-8 were fed with bMO diets at the designed doses above for 2 weeks prior to AOM, during and 1 week after DSS administration. At the end of the study, colon samples were processed for histopathological examination. PCNA indices, and iNOS and COX-2 expression were assessed by immunohistochemistry. The results demonstrated the incidences and multiplicities of tumors in Groups 6-8 to be decreased when compared to Group 2 in a dose dependent manner, but this was significant only in Group 8. The PCNA index was also significantly decreased in Group 8 whereas iNOS and COX-2 protein expression were significantly decreased in Groups 7 and 8. The findings suggest that M. oleifera Lam pod exerts suppressive effects in a colitis-related colon carcinogenesis model induced by AOM/DSS and could serve as a chemopreventive agent.
辣木（辣木树；嫩荚或果实）是泰国菜肴中的主要成分，具有一定的药用特性。先前的研究表明，其在体外和体内均具有潜在的抗氧化、抗肿瘤促进、抗断裂和抗癌活性。本研究旨在探讨辣木对小鼠由氧化偶氮甲烷（AOM）启动和葡聚糖硫酸钠（DSS）促进的结肠癌发生的化学预防作用。雄性ICR小鼠分为8组：第1组作为阴性对照；第2组接受AOM/DSS作为阳性对照；第3 - 5组分别在基础饮食中添加1.5%、3.0%和6.0%的水煮冻干辣木（bMO），持续5周；第6 - 8组在给予AOM前2周、给予DSS期间及之后1周，按上述设计剂量喂食bMO饮食。在研究结束时，对结肠样本进行组织病理学检查。通过免疫组织化学评估增殖细胞核抗原（PCNA）指数、诱导型一氧化氮合酶（iNOS）和环氧化酶 - 2（COX - 2）的表达。结果表明，与第2组相比，第6 - 8组肿瘤的发生率和多发性呈剂量依赖性降低，但仅在第8组有显著差异。第8组的PCNA指数也显著降低，而第7组和第8组的iNOS和COX - 2蛋白表达显著降低。研究结果表明，辣木荚在AOM/DSS诱导的结肠炎相关结肠癌发生模型中具有抑制作用，可作为化学预防剂。
