Wang Yuan, Hu Chaoying, Dong Ruofan, Huang Xiaoyan, Qiu Haifeng
Department of Obstetrics and Gynecology, the Fourth Affiliated Hospital of Soochow University, Wuxi, China.
Asian Pac J Cancer Prev. 2011;12(12):3367-70.
Platelet-derived growth factor-D (PDGF-D) can enhance invasion and metastasis in several human malignancies, though little is known about its functions in ovarian cancer.
In this study, we detected expression of PDGF-D in ovarian cancer tissues and cell lines by qRT-PCR, immunohistochemistry and western blotting, investigating the influences on cellular proliferation, invasion and apoptosis by upregulating its expression.
79.5% (62/78) of ovarian cancer samples proved to be PDGF-D positive, in contrast to just 38.5%(30/78) in their adjacent non-cancer tissues (p<0.001). Moreover, we found high levels of PDGF-D were correlated with lymph node metastasis (p=0.025) and positive cancer cells in abdominal washings/ascites (p=0.042). In vitro, upregulation of PDGF-D enhanced the invasiveness of SKOV3 cells (p<0.01), but had no impact on cellular proliferation or apoptosis. Furthermore, expression of matrix metalloproteinases 2/9 (MMP2 and MMP9) was positively related with PDGF-D, indicating their involvement in the invasion and metastasis of ovarian cancer.
Our findings proved that PDGF-D could promote ovarian cancer invasion by upregulating MMPs, which might be a potential target for ovarian cancer treatment.
血小板衍生生长因子-D（PDGF-D）可增强多种人类恶性肿瘤的侵袭和转移能力，不过其在卵巢癌中的功能鲜为人知。
在本研究中，我们通过qRT-PCR、免疫组织化学和蛋白质印迹法检测了卵巢癌组织和细胞系中PDGF-D的表达，通过上调其表达来研究对细胞增殖、侵袭和凋亡的影响。
79.5%（62/78）的卵巢癌样本被证明为PDGF-D阳性，相比之下，其相邻非癌组织中这一比例仅为38.5%（30/78）（p<0.001）。此外，我们发现高水平的PDGF-D与淋巴结转移（p=0.025）以及腹水中癌细胞阳性（p=0.042）相关。在体外，上调PDGF-D可增强SKOV3细胞的侵袭能力（p<0.01），但对细胞增殖或凋亡无影响。此外，基质金属蛋白酶2/9（MMP2和MMP9）的表达与PDGF-D呈正相关，表明它们参与了卵巢癌的侵袭和转移。
我们的研究结果证明，PDGF-D可通过上调基质金属蛋白酶促进卵巢癌侵袭，这可能是卵巢癌治疗的一个潜在靶点。
