Teng Kuo-Hsun, Liang Po-Huang
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Mol Membr Biol. 2012 Nov;29(7):267-73. doi: 10.3109/09687688.2012.674162. Epub 2012 Apr 4.
A group of prenyltransferases produce linear lipids by catalyzing consecutive condensation reactions of farnesyl diphosphate (FPP) with specific numbers of isopentenyl diphosphate (IPP), a common building block of isoprenoid compounds. Depending on the stereochemistry of the double bonds formed during IPP condensation, these prenyltransferases are categorized as cis- and trans-types. Undecaprenyl diphosphate synthase (UPPS) that catalyzes chain elongation of FPP by consecutive condensation reactions with eight IPP, to form C₅₅ lipid carrier for bacterial cell wall biosynthesis, serves as a model for understanding cis-prenyltransferases. In this review, the current knowledge in UPPS kinetics, mechanisms, structures, and inhibitors is summarized.
一组异戊二烯基转移酶通过催化法呢基二磷酸（FPP）与特定数量的异戊烯基二磷酸（IPP，类异戊二烯化合物的常见组成单元）的连续缩合反应来产生线性脂质。根据IPP缩合过程中形成的双键的立体化学结构，这些异戊二烯基转移酶可分为顺式和反式类型。十一异戊烯基二磷酸合酶（UPPS）通过与八个IPP连续缩合反应催化FPP的链延长，形成用于细菌细胞壁生物合成的C₅₅脂质载体，是理解顺式异戊二烯基转移酶的一个模型。在这篇综述中，总结了关于UPPS动力学、机制、结构和抑制剂的当前知识。
