Department of Biochemistry, Ege University, School of Medicine, 35100 Bornova, Izmir, Turkey.
J Inflamm (Lond). 2012 Apr 3;9:13. doi: 10.1186/1476-9255-9-13.
Recent findings suggest a role of oxidative stress in the pathogenesis of Behcet's disease (BD), but the utility of oxidative stress-associated assays in offering diagnostic information or in the monitoring of disease activity is largely unassessed.
We aimed to measure oxidative and inflammatory markers, along with the markers of reactive nitrogen species, S-nitrosothiols and 3-nitrotyrosine, in BD patients (n = 100) and healthy volunteers (n = 50). These markers were evaluated in regard to their role in the pathogenesis of BD as well as their relation to clinical presentation, disease activity and duration.
Median values for erythrocyte sedimentation rate (ESR), C-reactive protein, leukocyte count, and IL-18 levels, as well as myeloperoxidase (MPO) activity, were statistically higher in the patient group compared to controls. Some inflammation markers (ESR, neutrophil and leukocyte counts) were statistically higher (p < 0.05) in the active period. In contrast, oxidative stress-associated measures (erythrocyte lipid peroxidation, antioxidant enzymes and measures of serum antioxidant capacity), revealed no statistically significant differences between the median values in BD patients versus healthy control subjects (p > 0.05 in all statistical comparisons), nor was there any difference in median levels of these oxidative stress markers in active disease versus disease remission. S-nitrosothiols and 3-nitrotyrosine were undetectable in BD plasma.
The application of oxidative stress-associated measures to BD blood samples offered no supplemental diagnostic or disease activity information to that provided by standard laboratory measures of inflammation. S-nitrosothiols and 3-nitrotyrosine appeared not to be markers for active BD; thus the search for biochemical markers that will indicate the active period should be continued with larger studies.
最近的研究结果表明氧化应激在白塞病（BD）的发病机制中起作用，但氧化应激相关检测在提供诊断信息或监测疾病活动方面的效用在很大程度上尚未得到评估。
我们旨在测量 BD 患者（n=100）和健康志愿者（n=50）的氧化和炎症标志物，以及活性氮物种、S-亚硝基硫醇和 3-硝基酪氨酸的标志物。这些标志物的评估涉及它们在 BD 发病机制中的作用，以及它们与临床表现、疾病活动和病程的关系。
与对照组相比，患者组的红细胞沉降率（ESR）、C 反应蛋白、白细胞计数和 IL-18 水平以及髓过氧化物酶（MPO）活性的中位数均显著升高。一些炎症标志物（ESR、中性粒细胞和白细胞计数）在活动期升高更为显著（p<0.05）。相比之下，氧化应激相关的指标（红细胞脂质过氧化、抗氧化酶和血清抗氧化能力的指标）在 BD 患者与健康对照组之间的中位数没有统计学差异（所有统计比较的 p>0.05），在疾病活动期与缓解期之间，这些氧化应激标志物的中位数水平也没有差异。BD 患者的血浆中未检测到 S-亚硝基硫醇和 3-硝基酪氨酸。
将氧化应激相关指标应用于 BD 血样并没有提供比炎症标准实验室检测更有价值的补充诊断或疾病活动信息。S-亚硝基硫醇和 3-硝基酪氨酸似乎不是活动期 BD 的标志物；因此，应该继续进行更大规模的研究，寻找能够指示活动期的生化标志物。
