Cecchini Gualandi Stefano, Boni Raffaele
Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Antioxidants (Basel). 2023 Mar 12;12(3):701. doi: 10.3390/antiox12030701.
Simple and inexpensive analytical methods for assessing redox balance in biological matrixes are widely used in animal and human diagnostics. Two of them, reactive oxygen metabolites (ROMs) and total oxidant status (TOS), evaluate the lipid hydroperoxide (LOOH) content of the sample and are based on iron-mediated mechanisms. However, these tests provide uncorrelated results. In this study, we compared these two tests in the blood serum of goat kids and lambs, together with an evaluation of ceruloplasmin (CP) oxidase activity. No significant correlation was found between ROMs and TOS, or between TOS and CP oxidase activity, in either species. Conversely, ROMs and CP oxidase activity were highly correlated in both kid and lamb samples ( < 0.001). A significant progressive reduction in the analytical signal in the ROMs assay was observed when sodium azide, an effective CP inhibitor, was added to the samples before the assay ( < 0.001). This decrease was related to sodium azide concentration ( < 0.01) and was not found when sodium azide was added at the same concentrations in the TOS assay. These findings suggest that ROMs, unlike TOS, may be affected by CP, which interferes with LOOH detection in blood samples.
用于评估生物基质中氧化还原平衡的简单且廉价的分析方法广泛应用于动物和人类诊断。其中两种方法，即活性氧代谢产物（ROMs）和总氧化剂状态（TOS），评估样品中的脂质过氧化氢（LOOH）含量，且基于铁介导机制。然而，这些测试结果并不相关。在本研究中，我们比较了山羊幼崽和羔羊血清中的这两种测试方法，并评估了铜蓝蛋白（CP）氧化酶活性。在这两个物种中，ROMs与TOS之间，或TOS与CP氧化酶活性之间均未发现显著相关性。相反，在幼崽和羔羊样本中，ROMs与CP氧化酶活性均高度相关（<0.001）。在检测前向样本中加入有效的CP抑制剂叠氮化钠时，观察到ROMs检测中的分析信号显著逐步降低（<0.001）。这种降低与叠氮化钠浓度相关（<0.01），而在TOS检测中加入相同浓度的叠氮化钠时未发现这种情况。这些发现表明，与TOS不同，ROMs可能受CP影响，CP会干扰血液样本中LOOH的检测。
