Yoshida S, Senba E, Kubota Y, Hagihira S, Yoshiya I, Emson P C, Tohyama M
Department of Anatomy II, Osaka University Medical School, Japan.
Neuroscience. 1990;37(3):839-48. doi: 10.1016/0306-4522(90)90113-i.
Neurons containing the calcium-binding proteins, calbindin or parvalbumin, were studied by immunohistochemistry in the superficial dorsal horn of the rat spinal cord. Calbindin-containing cells were found in laminae I, II and III, being more abundant in laminae I and II. Some of the neurons in lamina I containing calbindin projected to the supraspinal area. Parvalbumin-containing neurons were mainly distributed in laminae IIi and III. Calbindin and parvalbumin were not detected in the same cells. Some 75% of the neurotensin-like immunoreactive neurons contained calbindin, which corresponded to 13% of the calbindin-containing neurons. Calbindin was sometimes found in the same cells with substance P, enkephalin or somatostatin but less frequently (44-46% of the peptide-containing neurons). Parvalbumin was not found together with these peptides. Electron microscopy showed that the immunoreactive products of calbindin or parvalbumin were mostly in the dendrites or cell bodies. Immunoreactive axon terminals were relatively few. In rhizotomized animals, neurons containing one of these proteins in laminae II and III were found to receive direct inputs of primary afferent fibers. These findings indicate that neurons containing these two proteins belong to different subpopulations of dorsal horn neurons. They may be important in primary afferent processing.
通过免疫组织化学方法，对大鼠脊髓浅表背角中含有钙结合蛋白钙结合蛋白或小白蛋白的神经元进行了研究。含钙结合蛋白的细胞见于Ⅰ、Ⅱ和Ⅲ层，在Ⅰ层和Ⅱ层更为丰富。Ⅰ层中一些含钙结合蛋白的神经元投射至上脊髓区域。含小白蛋白的神经元主要分布在Ⅱi层和Ⅲ层。在同一细胞中未检测到钙结合蛋白和小白蛋白。约75%的神经降压素样免疫反应性神经元含有钙结合蛋白，这相当于含钙结合蛋白神经元的13%。钙结合蛋白有时在与P物质、脑啡肽或生长抑素相同的细胞中发现，但频率较低（含肽神经元的44 - 46%）。未发现小白蛋白与这些肽共存。电子显微镜显示，钙结合蛋白或小白蛋白的免疫反应产物大多位于树突或细胞体中。免疫反应性轴突终末相对较少。在切断神经根的动物中，发现Ⅱ层和Ⅲ层中含有这些蛋白之一的神经元接受初级传入纤维的直接输入。这些发现表明，含有这两种蛋白的神经元属于背角神经元的不同亚群。它们在初级传入处理中可能很重要。
