Carr P A, Yamamoto T, Karmy G, Baimbridge K G, Nagy J I
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Neuroscience. 1989;33(2):363-71. doi: 10.1016/0306-4522(89)90216-9.
Histochemical and immunohistochemical techniques were used to determine relationships between the parvalbumin or calbindin D28k content and the cytochrome oxidase or carbonic anhydrase activity of neurons in lumbar dorsal root ganglia in rat. Subpopulations of dorsal root ganglion neurons that displayed parvalbumin- or calbindin D28k-immunoreactivity were classified as containing either light, moderate or dense histochemical reaction product for cytochrome oxidase and either a positive or negative reaction for carbonic anhydrase. It was found that approximately 90% of all parvalbumin and calbindin D28k-immunoreactive cells exhibited dense staining for cytochrome oxidase and that 87% of parvalbumin- and 76% of calbindin D28k-immunoreactive cells were positive for carbonic anhydrase. Conversely, 85% of all cells with a dense cytochrome oxidase reaction contained parvalbumin and calbindin D28k. Although not quantified, it appeared that many, but not all, carbonic anhydrase-positive cells contained parvalbumin or calbindin D28k. These results indicate the existence of a subpopulation of primary sensory neurons that contains parvalbumin and calbindin D28k and that expresses high levels of cytochrome oxidase and carbonic anhydrase activity. It is suggested that primary afferent neurons with this cytochemical profile transmit a sensory modality that requires them to discharge rapidly and/or frequently. The existence of a subpopulation of carbonic anhydrase-positive cells that lack immunoreactivity for parvalbumin or calbindin D28k suggests that the role of carbonic anhydrase in some sensory neurons is unrelated to functions requiring these calcium binding proteins.
采用组织化学和免疫组织化学技术来确定大鼠腰段背根神经节中神经元的小清蛋白或钙结合蛋白D28k含量与细胞色素氧化酶或碳酸酐酶活性之间的关系。将显示小清蛋白或钙结合蛋白D28k免疫反应性的背根神经节神经元亚群分类为细胞色素氧化酶组织化学反应产物为轻度、中度或重度，以及碳酸酐酶反应为阳性或阴性。结果发现，所有小清蛋白和钙结合蛋白D28k免疫反应性细胞中约90%对细胞色素氧化酶呈强染色，87%的小清蛋白免疫反应性细胞和76%的钙结合蛋白D28k免疫反应性细胞碳酸酐酶呈阳性。相反，所有细胞色素氧化酶反应强阳性的细胞中85%含有小清蛋白和钙结合蛋白D28k。虽然未进行量化，但似乎许多（但不是全部）碳酸酐酶阳性细胞含有小清蛋白或钙结合蛋白D28k。这些结果表明存在一群含有小清蛋白和钙结合蛋白D28k且表达高水平细胞色素氧化酶和碳酸酐酶活性的初级感觉神经元亚群。提示具有这种细胞化学特征的初级传入神经元传递一种需要它们快速和/或频繁放电的感觉模式。缺乏小清蛋白或钙结合蛋白D28k免疫反应性的碳酸酐酶阳性细胞亚群的存在表明，碳酸酐酶在一些感觉神经元中的作用与需要这些钙结合蛋白的功能无关。
