Mochizuki Kazuki, Misaki Yasumi, Miyauchi Rie, Takabe Satsuki, Shimada Masaya, Ichikawa Yoko, Goda Toshinao
Laboratory of Nutritional Physiology and Global COE Program, University of Shizuoka, Graduate School of Nutritional and Environmental Sciences.
J Nutr Sci Vitaminol (Tokyo). 2011;57(6):409-17. doi: 10.3177/jnsv.57.409.
Elevated circulating alanine aminotransferase (ALT) and γ-glutamyltranspeptidase (γ-GTP) activities in healthy and preclinical subjects are associated with increased risk for obesity, diabetes and related complications. In the present study, we examined the associations between these hepatic enzymes and circulating cytokines as markers for insulin sensitivity (adiponectin) and inflammation [interleukin-6 (IL-6)] in middle-aged Japanese men not being treated for metabolic diseases. We conducted a cross-sectional study of 310 Japanese men aged 40-69 y (mean ± SD, 58.8 ± 7.6 y) who were not being treated for metabolic diseases and who participated in health checkups in Japan. We analyzed their lifestyle factors, clinical factors, and plasma adiponectin and IL-6 concentrations. We determined associations between the concentrations of these cytokines and the clinical and lifestyle factors using Spearman's correlation analysis, Jonckheere-Terpstra's test and multiple linear regression. ALT activity was negatively associated with adiponectin (r=-0.302, p<0.001) but not with IL-6. γ-GTP activity was positively associated with IL-6 (r=0.335, p<0.001) and negatively associated with adiponectin (r=-0.129, p<0.05). Aspartate aminotransferase (AST) activity was positively associated with IL-6 (r=0.131, p<0.05) and negatively associated with adiponectin (r=-0.125, p<0.05). Multiple linear regression analyses showed that adiponectin was independently and negatively associated with ALT activity, while IL-6 was independently and positively associated with γ-GTP activity. Adiponectin and IL-6 were not independently associated with AST activity. The results of this study indicate that circulating ALT activity is negatively associated with adiponectin concentration, γ-GTP is positively associated with increased IL-6 concentration, and AST is not associated with these cytokines in middle-aged Japanese men not being treated for metabolic diseases.
健康和临床前受试者循环中丙氨酸氨基转移酶（ALT）和γ-谷氨酰转肽酶（γ-GTP）活性升高与肥胖、糖尿病及相关并发症风险增加有关。在本研究中，我们在未接受代谢疾病治疗的中年日本男性中，研究了这些肝酶与循环细胞因子之间的关联，这些细胞因子作为胰岛素敏感性（脂联素）和炎症[白细胞介素-6（IL-6）]的标志物。我们对310名年龄在40 - 69岁（平均±标准差，58.8±7.6岁）、未接受代谢疾病治疗且参加了日本健康检查的日本男性进行了横断面研究。我们分析了他们的生活方式因素、临床因素以及血浆脂联素和IL-6浓度。我们使用Spearman相关性分析、Jonckheere-Terpstra检验和多元线性回归确定了这些细胞因子浓度与临床和生活方式因素之间的关联。ALT活性与脂联素呈负相关（r = -0.302，p < 0.001），但与IL-6无关。γ-GTP活性与IL-6呈正相关（r = 0.335，p < 0.001），与脂联素呈负相关（r = -0.129，p < 0.05）。天冬氨酸氨基转移酶（AST）活性与IL-6呈正相关（r = 0.131，p < 0.05），与脂联素呈负相关（r = -0.125，p < 0.05）。多元线性回归分析表明，脂联素与ALT活性独立且呈负相关，而IL-6与γ-GTP活性独立且呈正相关。脂联素和IL-6与AST活性无独立关联。本研究结果表明，在未接受代谢疾病治疗的中年日本男性中，循环ALT活性与脂联素浓度呈负相关，γ-GTP与IL-6浓度升高呈正相关，而AST与这些细胞因子无关。
