Laboratory of Nutritional Physiology and Global COE Program, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.
Metabolism. 2011 Jul;60(7):914-22. doi: 10.1016/j.metabol.2010.08.011. Epub 2010 Oct 8.
Interleukin (IL)-1β and IL-6 expressions are known to be induced by oxidant stress. In the present study, we examined the relationships between these interleukins and the activity of γ-glutamyltranspeptidase (γ-GTP), which was recently reported as a source of oxidant stress production, in the circulating blood of middle-aged Japanese men without obvious cardiovascular diseases. We conducted a cross-sectional study of 317 Japanese men without obvious cardiovascular diseases aged 40 to 69 years (mean ± SD, 58.6 ± 7.6 years) who participated in health checkups in Japan. We analyzed their clinical parameters in serum, lifestyle factors, and plasma IL-1β and IL-6 concentrations. We compared the relationships between these interleukin concentrations and the clinical parameters and lifestyle factors by Spearman correlation coefficients. Stepwise multiple linear regression analyses for interleukins based on the other parameters and γ-GTP, which were classified into 3 groups according to the concentrations, were performed. Interleukin-1β and IL-6 concentrations were closely associated with γ-GTP activity but less associated with alanine aminotransferase and aspartate aminotransferase activities by Spearman correlation coefficients. Stepwise multiple linear regression analyses showed that γ-GTP activity was the explanatory variable for elevated IL-1β and IL-6 concentrations. As natural logarithms, the IL-1β and IL-6 concentrations were estimated to be 1.734- and 1.157-fold higher, respectively, in subjects with high γ-GTP activity ranges than in subjects with a low γ-GTP activity range. The present results show that circulating IL-1β and IL-6 concentrations are strongly and independently associated with γ-GTP activity in middle-aged Japanese men without obvious cardiovascular diseases.
白细胞介素 (IL)-1β 和 IL-6 的表达已知会被氧化应激诱导。在本研究中，我们检查了这些白细胞介素与γ-谷氨酰转肽酶 (γ-GTP) 活性之间的关系，γ-GTP 最近被报道为氧化应激产生的来源，在没有明显心血管疾病的中年日本男性循环血液中。我们对 317 名年龄在 40 至 69 岁（平均 ± 标准差，58.6 ± 7.6 岁）、无明显心血管疾病的日本男性进行了一项横断面研究，他们参加了日本的健康检查。我们分析了他们血清中的临床参数、生活方式因素以及血浆白细胞介素-1β 和白细胞介素-6 浓度。我们通过 Spearman 相关系数比较了这些白细胞介素浓度与临床参数和生活方式因素之间的关系。根据其他参数和 γ-GTP 进行了白细胞介素的逐步多元线性回归分析，γ-GTP 根据浓度分为 3 组。通过 Spearman 相关系数，白细胞介素-1β 和白细胞介素-6 浓度与 γ-GTP 活性密切相关，而与丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性的相关性较低。逐步多元线性回归分析表明，γ-GTP 活性是升高的白细胞介素-1β 和白细胞介素-6 浓度的解释变量。以自然对数表示，在 γ-GTP 活性高的受试者中，白细胞介素-1β 和白细胞介素-6 浓度分别估计比 γ-GTP 活性低的受试者高 1.734 倍和 1.157 倍。本研究结果表明，在无明显心血管疾病的中年日本男性中，循环白细胞介素-1β 和白细胞介素-6 浓度与 γ-GTP 活性强烈且独立相关。
