Laboratoire PMCN, Université de Lyon, Université Lyon1 and CNRS, UMR 5586, F-69622 Villeurbanne, France.
J Colloid Interface Sci. 2012 Jun 15;376(1):262-8. doi: 10.1016/j.jcis.2012.03.013. Epub 2012 Mar 14.
In this work, we study the adhesion forces between atomic force microscopy (AFM) tips and superficial dentin etched with phosphoric acid. Initially, we quantitatively analyze the effect of acid etching on the surface heterogeneity and the surface roughness, two parameters that play a key role in the adhesion phenomenon. From a statistical study of the force-distance curves, we determine the average adhesion forces on the processed substrates. Our results show that the average adhesion forces, measured in water, increase linearly with the acid exposure time. The highest values of such forces are ascribed to the high density of collagen fibers on the etched surfaces. The individual contribution of exposed collagen fibrils to the adhesion force is highlighted. We also discuss in this paper the influence of the environmental medium (water/air) in the adhesion measurements. We show that the weak forces involved require working in the aqueous medium.
在这项工作中，我们研究了原子力显微镜（AFM）探针与磷酸蚀刻后的表面牙本质之间的粘附力。最初，我们定量分析了酸蚀对表面不均匀性和表面粗糙度的影响，这两个参数在粘附现象中起着关键作用。通过对力-距离曲线的统计研究，我们确定了处理后的基底上的平均粘附力。我们的结果表明，在水中测量的平均粘附力随酸暴露时间呈线性增加。这些力的最高值归因于蚀刻表面上胶原纤维的高密度。突出显示了暴露的胶原原纤维对粘附力的个体贡献。本文还讨论了环境介质（水/空气）对粘附测量的影响。我们表明，涉及的弱力需要在水介质中工作。
