Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, University of Copenhagen, Denmark.
Br J Cancer. 2012 Apr 24;106(9):1526-34. doi: 10.1038/bjc.2012.109.
Although the role of human papilloma virus (HPV) in cervical squamous cell carcinoma (CSCC) is well established, the role in head and neck SCC (HNSCC) is less clear. MicroRNAs (miRNAs) have a role in the cancer development, and HPV status may affect the miRNA expression pattern in HNSCC. To explore the influence of HPV in HNSCC, we made a comparative miRNA profile of HPV-positive (HPV+) and HPV-negative (HPV-) HNSCC against CSCC.
Fresh frozen and laser microdissected-paraffin-embedded samples obtained from patients with HPV+/HPV- HNSCC, CSCC and controls were used for microarray analysis. Differentially expressed miRNAs in the HPV+ and HPV- HNSCC samples were compared with the differentially expressed miRNAs in the CSCC samples.
Human papilloma virus positive (+) HNSCC had a distinct miRNA profile compared with HPV- HNSCC. Significantly more similarity was seen between HPV+ HNSCC and CSCC than HPV- and CSCC. A set of HPV core miRNAs were identified. Of these especially the miR-15a/miR-16/miR195/miR-497 family, miR-143/miR-145 and the miR-106-363 cluster appear to be important within the known HPV pathogenesis.
This study adds new knowledge to the known pathogenic pathways of HPV and substantiates the oncogenic role of HPV in subsets of HNSCCs.
尽管人乳头瘤病毒（HPV）在宫颈鳞状细胞癌（CSCC）中的作用已得到充分证实，但在头颈部鳞状细胞癌（HNSCC）中的作用尚不清楚。微小 RNA（miRNA）在癌症发展中起作用，HPV 状态可能影响 HNSCC 中的 miRNA 表达模式。为了探讨 HPV 在 HNSCC 中的作用，我们对 HPV 阳性（HPV+）和 HPV 阴性（HPV-）HNSCC 与 CSCC 进行了比较 miRNA 谱分析。
使用来自 HPV+/HPV-HNSCC、CSCC 和对照患者的新鲜冷冻和激光显微切割-石蜡包埋样本进行微阵列分析。比较 HPV+和 HPV-HNSCC 样本中差异表达的 miRNA 与 CSCC 样本中差异表达的 miRNA。
HPV 阳性（+）HNSCC 与 HPV-HNSCC 相比具有独特的 miRNA 谱。HPV+ HNSCC 与 CSCC 之间的相似性明显高于 HPV-和 CSCC 之间的相似性。鉴定出一组 HPV 核心 miRNA。其中，miR-15a/miR-16/miR195/miR-497 家族、miR-143/miR-145 和 miR-106-363 簇似乎在已知的 HPV 发病机制中非常重要。
本研究为 HPV 已知发病途径增加了新知识，并证实了 HPV 在 HNSCC 亚群中的致癌作用。
