Department of Anatomy, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Šrobárova Street 2, 041 80 Košice, Slovakia.
Eur J Histochem. 2012 Feb 8;56(1):e7. doi: 10.4081/ejh.2012.e7.
The aim of this work was to determine the localization of nicotinamide-adenine dinucleotide phosphate-diaphorase (NADPH-d) activity as the marker for synthesis of nitric oxide synthase (NOS) in the rat thymus after the application of retinoid acid (RA) on 1st, 7th, 14th and 21st days of gestation. The given results can build the basis for understanding of the role of NOS in rat thymus. NADPH-d positive cells were represented with dark-blue color and were localized on corticomedullar junction of the thymus. These cells were of different intensity of coloring and were shaped in oval, circle or irregular forms. NADPH-d positive nerve fibers were observed in perivascular topography. They were marked more strongly in the case of control group. The result of application of RA to gravid rats was that the birth weights of newborn rats and their thymuses were smaller, but without statistically significance.
本工作旨在确定烟酰胺腺嘌呤二核苷酸磷酸-黄递酶 (NADPH-d) 活性的定位，作为在妊娠第 1、7、14 和 21 天应用维甲酸 (RA) 后大鼠胸腺中一氧化氮合酶 (NOS) 合成的标志物。所提供的结果可以为理解 NOS 在大鼠胸腺中的作用奠定基础。NADPH-d 阳性细胞呈深蓝色，表示位于胸腺皮质髓质交界处。这些细胞的着色强度不同，呈椭圆形、圆形或不规则形状。NADPH-d 阳性神经纤维在血管周围分布。对照组的标记更为强烈。将 RA 应用于妊娠大鼠的结果是，新生大鼠及其胸腺的出生体重较小，但无统计学意义。
