Universidade de São Paulo, São Paulo, SP, Brazil.
Braz Oral Res. 2012 Mar-Apr;26(2):139-44. doi: 10.1590/s1806-83242012000200009.
Little is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.
关于牙源性黏液瘤（OM）的组织发生，人们知之甚少。牙髓干细胞可能是 OM 的候选前体细胞，因为 OM 和牙髓具有相同的胚胎起源：牙髓乳头。为了比较 OM 和干细胞，本研究分析了与 OM 侵袭性相关的两种蛋白质（MMP-2 和透明质酸）在人未成熟牙髓干细胞（hIDPSCs）中的表达。本研究使用了来自乳牙和恒牙的三种 hIDPSC 谱系。免疫荧光显示所有 hIDPSCs 均对 MMP-2 和透明质酸 (HA) 呈阳性反应。MMP-2 出现在整个细胞质中的小点，而 HA 则出现在整个细胞质和细胞体外部的弥散和不规则点或短纤维中。使用 RT-PCR 分析评估每个细胞谱系的基因表达谱，结果表明 HA 的表达比 MMP-2 更强烈。HA 的表达在三种 hIDPSC 谱系中相似，而 MMP-2 在 DL-1 中的表达高于其他细胞系。hIDPSCs 中与 OM 侵袭性相关的蛋白质的表达表明 OM 来源于牙髓干细胞。
